Multidrug-resistant Raoultella ornithinolytica misidentified as Klebsiella oxytoca carrying blaOXA ß-lactamases: antimicrobial profile and genomic characterization
Por:
Cortés-Ortíz I.A., Mendieta-Condado E., Escobar-Escamilla N., Juárez-Gómez J.C., Garcés-Ayala F., Rodriguez A.A., Bravata-Alcántara J.C., Gutiérrez-Muñoz V.H., Bello-López J.M., Ramírez–González J.E.
Publicada:
1 ene 2021
Resumen:
Class D ß-lactamases OXA-232 and OXA-48 hydrolyze penicillin, cephalosporins and carbapenems, limiting the pharmacological therapeutics in bacteraemia. OXA producer microorganisms are considered a great emergent threat, especially in nosocomial environments. To determine the resistance profile and genomic characterization of two isolates initially identified as potential carbapenemase-producer Klebsiella oxytoca in a third level hospital. Automated platform BD Phoenix-100 System was used to identify and to biochemically characterize both isolates. Furthermore, the resistance profile was determined through CLSI methods and the whole genome sequences were obtained using Next-Generation Sequencing. Resistance genes were analyzed, and the virtual fingerprinting was determined to corroborate the similarity with related bacteria. Both strains correspond to Raoultella ornithinolytica carrying OXA 232 and OXA-48 genes, confirming the class D ß-lactamases assay results. Here, we present the genetic and phenotypic analysis of multidrug resistance R. ornithinolytica, representing the first report in Mexico. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Filiaciones:
Laboratorio de Genética y Diagnóstico Molecular, Hospital Juárez de México. Secretaria de Salud, CDMX, Mexico
Unidad de Desarrollo Tecnológico e Investigación Molecular, Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) “Dr. Manuel Martínez Báez”. Secretaria de Salud, Francisco de P. Miranda 177, Lomas de Plateros, Álvaro Obregón, CDMX, 01480, Mexico
Laboratorio de Bacteriología., Laboratorio Central. Hospital Juárez de México. Secretaria de Salud, CDMX, Mexico
Vigilancia Epidemiológica., Hospital Juárez de México. Secretaria de Salud, CDMX, Mexico
División de Investigación., Hospital Juárez de México. Secretaria de Salud, CDMX, Mexico
|