A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation
Por:
Das S., Bernasconi E., Koutsokera A., Wurlod D.-A., Tripathi V., Bonilla-Rosso G., Aubert J.-D., Derkenne M.-F., Mercier L., Pattaroni C., Rapin A., von Garnier C., Marsland B.J., Engel P., Nicod L.P.
Publicada:
1 ene 2021
Resumen:
There is accumulating evidence that the lower airway microbiota impacts lung health. However, the link between microbial community composition and lung homeostasis remains elusive. We combine amplicon sequencing and bacterial culturing to characterize the viable bacterial community in 234 longitudinal bronchoalveolar lavage samples from 64 lung transplant recipients and establish links to viral loads, host gene expression, lung function, and transplant health. We find that the lung microbiota post-transplant can be categorized into four distinct compositional states, ‘pneumotypes’. The predominant ‘balanced’ pneumotype is characterized by a diverse bacterial community with moderate viral loads, and host gene expression profiles suggesting immune tolerance. The other three pneumotypes are characterized by being either microbiota-depleted, or dominated by potential pathogens, and are linked to increased immune activity, lower respiratory function, and increased risks of infection and rejection. Collectively, our findings establish a link between the lung microbial ecosystem, human lung function, and clinical stability post-transplant. © 2021, The Author(s).
Filiaciones:
Das S.:
Department of Fundamental Microbiology, Biophore, University of Lausanne, Lausanne, Switzerland
Bernasconi E.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Koutsokera A.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Wurlod D.-A.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Tripathi V.:
Department of Fundamental Microbiology, Biophore, University of Lausanne, Lausanne, Switzerland
Biozentrum, University of Basel, Basel, Switzerland
Bonilla-Rosso G.:
Department of Fundamental Microbiology, Biophore, University of Lausanne, Lausanne, Switzerland
Aubert J.-D.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Derkenne M.-F.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Mercier L.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Pattaroni C.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, VIC, Australia
Rapin A.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
von Garnier C.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Marsland B.J.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, VIC, Australia
Engel P.:
Department of Fundamental Microbiology, Biophore, University of Lausanne, Lausanne, Switzerland
Nicod L.P.:
Department of Respiratory Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland
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