Heparin and low molecular weight heparin decrease nitric oxide production by human polymorphonuclear cells
Por:
Beltrán A.E., Concepción F., Manzanares D., Garrido G., Glaria L.A., Rojas A.
Publicada:
1 ene 1999
Resumen:
Background. Heparin and heparin derivatives with low anticoagulant activity exhibit a wide spectrum of biological functions affecting adhesion, activation and trafficking of leukocytes. Methods. We investigated the in vitro effect of heparin and a low molecular weight heparin derivative (LMWH) on nitric oxide (NO) production by human polymorphonuclear leukocytes (PMN). Results. N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated NO production was significantly decreased by heparin at doses of 0.5 and 5 µg/mL, while LMWH was only effective at doses of 50 and 200 µg/mL by means of a mechanism not related to NO synthase (NOS) activity. Coclusions. These results support the hypothesis that heparin and LMWH derivatives may offer therapeutic benefit for inflammatory diseases where NO plays a protagonic role.
Filiaciones:
Beltrán A.E.:
Laboratorio de Farmacología, Ctro. de Quim. Farmaceut., la, Habana, Cuba
Concepción F.:
Laboratorio de Análisis, Ctro. de Quim. Farmaceut., la, Habana, Cuba
Manzanares D.:
Ctro. de Invest. Biomédicas, La, Habana, Cuba
Garrido G.:
Laboratorio de Farmacología, Ctro. de Quim. Farmaceut., la, Habana, Cuba
Glaria L.A.:
Laboratorio de Farmacología, Ctro. de Quim. Farmaceut., la, Habana, Cuba
Rojas A.:
Laboratorio de Farmacología, Ctro. de Quim. Farmaceut., la, Habana, Cuba
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