LIPID transfer proteins regulate store-operated calcium entry via control of plasma membrane phosphoinositides
Por:
Gulyas, Gergo, Korzeniowski, Marek K., Eugenio, Carlos Ernesto Bastian, Vaca, Luis, Kim, Yeun Ju, Balla, Tamas
Publicada:
1 sep 2022
Resumen:
The ER-resident proteins STIM1 together with the plasma membrane
(PM)-localized Orai1 channels constitute the molecular components of the
store-operated Ca2+ entry (SOCE) pathway. Prepositioning of STIM1 to the
peripheral ER close to the PM ensures its efficient interaction with
Orail upon a decrease in the ER luminal Ca2+ concentration. The
C-terminal polybasic domain of STIM1 has been identified as mediating
the interaction with PM phosphoinositides and hence positions the
molecule to ER-PM contact sites. Here we show that STIM1 requires PM
phosphatidylinositol 4-phosphate (PI4P) for efficient PM interaction.
Accordingly, oxysterol binding protein related proteins (ORPs) that work
at ER-PM junctions and consume PI4P gradients exert important control
over the Ca2+ entry process. These studies reveal an important
connection between non-vesicular lipid transport at ER-PM contact sites
and regulation of ER Ca2+ store refilling.
Filiaciones:
Gulyas, Gergo:
Eunice Kennedy Shriver NICHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD USA
Korzeniowski, Marek K.:
Eunice Kennedy Shriver NICHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD USA
Uniformed Serv Univ Hlth Sci, Dept Anat Physiol & Genet, Bethesda, MD USA
Eugenio, Carlos Ernesto Bastian:
Eunice Kennedy Shriver NICHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD USA
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Vaca, Luis:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Kim, Yeun Ju:
Eunice Kennedy Shriver NICHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD USA
Balla, Tamas:
Eunice Kennedy Shriver NICHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD USA
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