La desnutrición crónica en ratas causada por alimentación a base de maíz aumenta la susceptibilidad a convulsiones experimentalmente inducidas por la administración de L-glutamato monosódico
Por:
Feria-Velasco A., Orozco-Suárez S., Cárabez-Torres J., Del Angel A.R., Beas-Zárate C.
Publicada:
1 ene 2006
Resumen:
Introduction: feeding rats with a corn-based diet (deficient in tryptophan, lysine and protein) before and during pregnancy has been associated with low brain serotonin synthesis and fewer cortical GABAergic neurons in offspring than in animals fed with normal Purina Chow (CTRL group), altering brain excitability. On the other hand, the systemic injection of a hyperosmolar solution of monosodium L-glutamate (MSG) to rats represents a valuable model of status epilepticus. Objective: this work was oriented to investigate whether cerebral changes induced by feeding rats a corn-based diet (prenatal and postnatally), might be associated with more susceptibility to MSG-induced seizures in a well-established model of status epilepticus. Materials and methods: chronic malnutrition was induced in adult female rats with a corn-based diet (CORN group) before and during pregnancy and the males from their offspring continued with the same diet until becoming 60-days old. The pattern and severity of convulsive phenomenon induced by various doses of MSG were evaluated in these animals. As the corn diet contains a low proportion of protein (8%), a group of animals under a hypoproteic diet was included as a matched control group (HYP), while a group of animals fed a normal Chow Purina diet was included as a normal control group (CTRL). Results: animals from CORN and HYP groups showed a greater susceptibility to convulsions than those fed a CTRL diet. The most severe effect was seen in rats from the CORN group. Conclusion: the results obtained are directly related to differences in the nutrient content of diets (especially TRY and protein), which are probably associated with alterations in the inhibitory neurotransmitter systems regulating brain excitability. ©INNN, 2006.
Filiaciones:
Feria-Velasco A.:
Unidad de Morfología de Alta Resolución, Centro Universitario de Ciencias Biológicas y Agropecuarias (CUCBA), Universidad de Guadalajara, Las Agujas, Mexico
Unidad de Morfología de Alta Resolución, CUCBA, Universidad de Guadalajara, Km. 15.5 Carr. Guad.-Nogales, 45100 Las Agujas, Nextipac, Zapopan, Mexico
Orozco-Suárez S.:
Unidad de Investigación Médica en Enfermedades Neurológicas, CMN, Institituto Mexicano del Seguro Social (MSS), México D.F., Mexico
Cárabez-Torres J.:
Depto. de Producción Animal, CUCBA, Universidad de Guadalajara, México, Mexico
Del Angel A.R.:
Division de Investigación en Neurociencias, Centro de Investigación Biomédica de Occidente, IMSS, Guadalajara, Jal., Mexico
Beas-Zárate C.:
Depto. de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias (CUCBA), Universidad de Guadalajara, Las Agujas, Mexico
Division de Investigación en Neurociencias, Centro de Investigación Biomédica de Occidente, IMSS, Guadalajara, Jal., Mexico
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