Alternative CUG Codon Usage in the Halotolerant Yeast Debaryomyces hansenii: Gene Expression Profiles Provide New Insights into Ambiguous Translation
Por:
Ochoa-Gutierrez, Daniel, Reyes-Torres, Anya M., de la Fuente-Colmenares, Ileana, Escobar-Sanchez, Viviana, Gonzalez, James, Ortiz-Hernandez, Rosario, Torres-Ramirez, Nayeli, Segal-Kischinevzky, Claudia
Publicada:
1 sep 2022
Resumen:
The halotolerant yeast Debaryomyces hansenii belongs to the CTG-Ser1
clade of fungal species that use the CUG codon to translate as leucine
or serine. The ambiguous decoding of the CUG codon is relevant for
expanding protein diversity, but little is known about the role of
leucine-serine ambiguity in cellular adaptations to extreme
environments. Here, we examine sequences and structures of tRNA(CAG)
from the CTG-Ser1 clade yeasts, finding that D. hansenii conserves the
elements to translate ambiguously. Then, we show that D. hansenii has
tolerance to conditions of salinity, acidity, alkalinity, and oxidative
stress associated with phenotypic and ultrastructural changes. In these
conditions, we found differential expression in both the logarithmic and
stationary growth phases of tRNA(Ser), tRNA(Leu), tRNA(CAG), LeuRS, and
SerRS genes that could be involved in the adaptive process of this
yeast. Finally, we compare the proteomic isoelectric points and
hydropathy profiles, detecting that the most important variations among
the physicochemical characteristics of D. hansenii proteins are in their
hydrophobic and hydrophilic interactions with the medium. We propose
that the ambiguous translation, i.e., leucylation or serynation, on
translation of the CUG-encoded residues, could be linked to adaptation
processes in extreme environments.
Filiaciones:
Ochoa-Gutierrez, Daniel:
Univ Nacl Autonoma Mexico, Lab Biol Mol & Genom, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Ciencias Biol, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Reyes-Torres, Anya M.:
Univ Nacl Autonoma Mexico, Lab Biol Mol & Genom, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
de la Fuente-Colmenares, Ileana:
Univ Nacl Autonoma Mexico, Lab Biol Mol & Genom, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Escobar-Sanchez, Viviana:
Univ Nacl Autonoma Mexico, Lab Biol Mol & Genom, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Gonzalez, James:
Univ Nacl Autonoma Mexico, Lab Biol Mol & Genom, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Ortiz-Hernandez, Rosario:
Univ Nacl Autonoma Mexico, Lab Microscopia Elect, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Torres-Ramirez, Nayeli:
Univ Nacl Autonoma Mexico, Lab Microscopia Elect, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Segal-Kischinevzky, Claudia:
Univ Nacl Autonoma Mexico, Lab Biol Mol & Genom, Fac Ciencias, Dept Biol Celular, Ave Univ 3000,Cd Univ, Mexico City 04510, DF, Mexico
Green Published, gold
|