Biologically synthesized silver nanoparticles as potent antibacterial effective against multidrug-resistant Pseudomonas aeruginosa
Por:
Campo-Beleno, C., Villamizar-Gallardo, R. A., Lopez-Jacome, L. E., Gonzalez, E. E., Munoz-Carranza, S., Franco, B., Morales-Espinosa, R., Coria-Jimenez, R., Franco-Cendejas, R., Hernandez-Duran, M., Lara-Martinez, R., Jimenez-Garcia, L. F., Fernandez-Presas, A. M., Garcia-Contreras, R.
Publicada:
1 sep 2022
Categoría:
Applied microbiology and biotechnology
Resumen:
Pseudomonas aeruginosa is one of the most worrisome infectious bacteria
due to its intrinsic and acquired resistance against several antibiotics
and the recalcitrance of its infections; hence, the development of novel
antimicrobials effective against multidrug-resistant P. aeruginosa is
mandatory. In this work, silver nanoparticles obtained by green
synthesis using a leaf extract and fungi were tested against a battery
of clinical strains from cystic fibrosis, pneumonia and burnt patients,
some of them with multidrug resistance. Both nanoparticles showed a
potent antibacterial effect, causing severe damage to the cell wall,
membrane and DNA, and inducing the production of reactive oxygen
species. Moreover, the nanoparticles derived from fungi showed
synergistic antibacterial effects with the antibiotics meropenem and
levofloxacin for some clinical strains and both kinds of nanoparticles
were nontoxic for larvae of the moth Galleria mellonella, encouraging
further research for their implementation in the treatment of P.
aeruginosa infections.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Univ Pamplona, Fac Salud, Dept Med, Cucuta, Norte De Santan, Colombia
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Infect Dis Div, Mexico City, DF, Mexico
Ctr Ciencia & Tecnol Nanoescalar, NanCiTec, Bogota, Colombia
Univ Guanajuato, Dept Biol, Div Ciencias Nat & Exactas, Guanajuato, Mexico
Inst Nacl Pediat, Lab Bacteriol Expt, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, UNAM, Mexico City, DF, Mexico
Fernandez-Presas, AM (Corresponding Author), Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
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