Herpesvirus Screening in Childhood Hematopoietic Transplant Reveals High Systemic Inflammation in Episodes of Multiple Viral Detection and an EBV Association with Elevated IL-1 beta, IL-8 and Graft-Versus-Host Disease
Por:
Rojas-Rechy, Moises H., Gaytan-Morales, Felix, Sanchez-Ponce, Yessica, Castorena-Villa, Ivan, Lopez-Martinez, Briceida, Parra-Ortega, Israel, Escamilla-Nunez, Maria C., Mendez-Tenorio, Alfonso, Pompa-Mera, Ericka N., Martinez-Ruiz, Gustavo U., Fuentes-Panana, Ezequiel M., Morales-Sanchez, Abigail
Publicada:
1 ago 2022
Resumen:
Infections remain a major cause of morbidity and mortality among
hematopoietic stem cell transplant (HSCT) recipients. Unlike
Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV), Human
Herpesvirus (HHV) 6, HHV7 and HHV8 are not routinely monitored in many
centers, especially in the pediatric population of low-medium income
countries. We screened EBV, HCMV, HHV6, HHV7 and HHV8 in 412
leukocytes-plasma paired samples from 40 pediatric patients assisted in
a tertiary hospital in Mexico. Thirty-two underwent allo-HSCT, whereas
eight received auto-HSCT. Overall viral detection frequencies in allo-
and auto-HSCT were: EBV = 43.7% and 30.0%, HCMV = 5.0% and 6.7%,
HHV6 = 7.9% and 20.0% and HHV7 = 9.7% and 23.3%. HHV8 was not
detected in any sample. Interestingly, HHV6 and HHV7 were more frequent
in auto-HSCT, and HHV6 was observed in all episodes of multiple
detection in auto-HSCT patients. We found EBV DNA in plasma samples,
whereas HCMV, HHV6 and HHV7 DNA were predominantly observed in
leukocytes, indicative of their expansion in cellular compartments. We
also found that IL-1 beta, IL-2, IL-6 and IL-8 were significantly
increased in episodes in which multiple viruses were simultaneously
detected, and samples positive for EBV DNA and graft-versus-host disease
had a further increase of IL-1 beta and IL-8. In conclusion, the EBV,
HCMV, HHV6 and HHV7 burdens were frequently detected in allo- and
auto-HSCT, and their presence associated with systemic inflammation.
Filiaciones:
Rojas-Rechy, Moises H.:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
Natl Polytech Inst, Postgrad Program Biomed & Mol Biotechnol, Mexico City 11340, DF, Mexico
Gaytan-Morales, Felix:
Childrens Hosp Mexico Federico Gomez, Bone Marrow Transplant Serv, Mexico City 06720, DF, Mexico
Sanchez-Ponce, Yessica:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
Castorena-Villa, Ivan:
Childrens Hosp Mexico Federico Gomez, Bone Marrow Transplant Serv, Mexico City 06720, DF, Mexico
Lopez-Martinez, Briceida:
Childrens Hosp Mexico Federico Gomez, Subdirect Diagnost Auxiliary Serv, Mexico City 06720, DF, Mexico
Parra-Ortega, Israel:
Childrens Hosp Mexico Federico Gomez, Dept Clin Lab, Mexico City 06720, DF, Mexico
Escamilla-Nunez, Maria C.:
Natl Inst Publ Hlth, Cuernavaca 62100, Morelos, Mexico
Mendez-Tenorio, Alfonso:
Inst Politecn Nacl, Lab Biotecnol & Bioinformat Genom, Escuela Nacl Ciencias Biol, Mexico City 11340, DF, Mexico
Pompa-Mera, Ericka N.:
Mexican Inst Social Secur IMSS, Natl Med Ctr CMN SIGLO XXI, Pediat Hosp, Infect & Parasit Dis Med Res Unit UIMEIP, Mexico City 06720, DF, Mexico
Martinez-Ruiz, Gustavo U.:
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Mexico City 04510, DF, Mexico
Childrens Hosp Mexico Federico Gomez, Lab Invest Patol Expt, Mexico City 06720, DF, Mexico
Fuentes-Panana, Ezequiel M.:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
Morales-Sanchez, Abigail:
Childrens Hosp Mexico Federico Gomez, Res Unit Virol & Canc, Mexico City 06720, DF, Mexico
Green Accepted, gold, All Open Access, Gold, Green
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