Evaluation of the Biocompatibility and Osteogenic Properties of Metal Oxide Coatings Applied by Magnetron Sputtering as Potential Biofunctional Surface Modifications for Orthopedic Implants
Por:
Fernandez-Lizarraga, Mariana, Garcia-Lopez, Julieta, Rodil, Sandra E., Maria Ribas-Aparicio, Rosa, Silva-Bermudez, Phaedra
Publicada:
1 ago 2022
Resumen:
Biomaterials with adequate properties to direct a biological response
are essential for orthopedic and dental implants. The surface properties
are responsible for the biological response; thus, coatings with
biologically relevant properties such as osteoinduction are exciting
options to tailor the surface of different bulk materials. Metal oxide
coatings such as TiO2, ZrO2, Nb2O5 and Ta2O5 have been suggested as
promising for orthopedic and dental implants. However, a comparative
study among them is still missing to select the most promising for
bone-growth-related applications. In this work, using magnetron
sputtering, TiO2, ZrO2, Ta2O5, and Nb2O5 thin films were deposited on Si
(100) substrates. The coatings were characterized by Optical
Profilometry, Scanning Electron Microscopy, Energy-Dispersive X-ray
Spectroscopy, X-ray Photoelectron Spectroscopy, X-ray Diffraction, Water
Contact Angle measurements, and Surface Free Energy calculations. The
cell adhesion, viability, proliferation, and differentiation toward the
osteoblastic phenotype of mesenchymal stem cells plated on the coatings
were measured to define the biological response. Results confirmed that
all coatings were biocompatible. However, a more significant number of
cells and proliferative cells were observed on Nb2O5 and Ta2O5 compared
to TiO2 and ZrO2. Nevertheless, Nb2O5 and Ta2O5 seemed to induce cell
differentiation toward the osteoblastic phenotype in a longer cell
culture time than TiO2 and ZrO2.
Filiaciones:
Fernandez-Lizarraga, Mariana:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Posgrad Doctorado Ciencias Biomed & Biotecnol Mol, Mexico City 11340, DF, Mexico
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Unidad Ingn Tejidos Terapia Celular & Med Regener, Mexico City 14389, DF, Mexico
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Lab Prod & Control Biol, Mexico City 11340, DF, Mexico
Garcia-Lopez, Julieta:
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Unidad Ingn Tejidos Terapia Celular & Med Regener, Mexico City 14389, DF, Mexico
Rodil, Sandra E.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
Maria Ribas-Aparicio, Rosa:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Posgrad Doctorado Ciencias Biomed & Biotecnol Mol, Mexico City 11340, DF, Mexico
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Lab Prod & Control Biol, Mexico City 11340, DF, Mexico
Silva-Bermudez, Phaedra:
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Unidad Ingn Tejidos Terapia Celular & Med Regener, Mexico City 14389, DF, Mexico
gold, Green Accepted, Green Published, Gold, Green
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