Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
Por:
Ibarra-Laclette, Enrique, Venancio-Rodriguez, Carlos Ariel, Vasquez-Aguilar, Antonio Acini, Alonso-Sanchez, Alexandro G., Perez-Torres, Claudia-Anahi, Villafan, Emanuel, Ramirez-Barahona, Santiago, Galicia, Sonia, Sosa, Victoria, Rebollar, Eria A., Lara, Carlos, Gonzalez-Rodriguez, Antonio, Diaz-Fleisher, Francisco, Ornelas, Juan Francisco
Publicada:
13 jun 2022
Resumen:
The mistletoe Psittacanthus schiedeanus, a keystone species in
interaction networks between plants, pollinators, and seed dispersers,
infects a wide range of native and non-native tree species of commercial
interest. Here, using RNA-seq methodology we assembled the whole
circularized quadripartite structure of P. schiedeanus chloroplast
genome and described changes in the gene expression of the nuclear
genomes across time of experimentally inoculated seeds. Of the 140,467
assembled and annotated uniGenes, 2,000 were identified as
differentially expressed (DEGs) and were classified in six distinct
clusters according to their expression profiles. DEGs were also
classified in enriched functional categories related to synthesis,
signaling, homoeostasis, and response to auxin and jasmonic acid. Since
many orthologs are involved in lateral or adventitious root formation in
other plant species, we propose that in P. schiedeanus (and perhaps in
other rootless mistletoe species), these genes participate in haustorium
formation by complex regulatory networks here described. Lastly, and
according to the structural similarities of P. schiedeanus enzymes with
those that are involved in host cell wall degradation in fungi, we
suggest that a similar enzymatic arsenal is secreted extracellularly and
used by mistletoes species to easily parasitize and break through
tissues of the host.
Filiaciones:
Ibarra-Laclette, Enrique:
Inst Ecol AC INECOL, Red Estudios Mol Avanzados REMAv, Xalapa, Mexico
Venancio-Rodriguez, Carlos Ariel:
Inst Ecol AC INECOL, Red Estudios Mol Avanzados REMAv, Xalapa, Mexico
Vasquez-Aguilar, Antonio Acini:
Inst Ecol AC INECOL, Red Biol Evolut, Xalapa, Mexico
Alonso-Sanchez, Alexandro G.:
Inst Ecol AC INECOL, Red Estudios Mol Avanzados REMAv, Xalapa, Mexico
Perez-Torres, Claudia-Anahi:
Inst Ecol AC INECOL, Red Estudios Mol Avanzados REMAv, Xalapa, Mexico
Investigador Mexico CONACyT Inst Ecol A C INECOL, Xalapa, Mexico
Villafan, Emanuel:
Inst Ecol AC INECOL, Red Estudios Mol Avanzados REMAv, Xalapa, Mexico
Ramirez-Barahona, Santiago:
Univ Nacl Autonoma Mexico, Dept Bot, Inst Biol, Mexico City, Mexico
Galicia, Sonia:
Inst Ecol AC INECOL, Red Biol Evolut, Xalapa, Mexico
Sosa, Victoria:
Inst Ecol AC INECOL, Red Biol Evolut, Xalapa, Mexico
Rebollar, Eria A.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca, Mexico
Lara, Carlos:
Univ Autonoma Tlaxcala, Ctr Invest Ciencias Biol, Tlaxcala, Mexico
Gonzalez-Rodriguez, Antonio:
UNAM, Inst Invest Ecosistemas & Sustentabil IIES, Lab Genet Conservac, Morelia, Mexico
Diaz-Fleisher, Francisco:
Univ Veracruzana, INBIOTECA, Xalapa, Mexico
Ornelas, Juan Francisco:
Inst Ecol AC INECOL, Red Biol Evolut, Xalapa, Mexico
gold, Green Published, All Open Access, Gold, Green
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