Annexin in Taenia crassiceps ORF Strain is Localized in the Osmoregulatory System
Por:
Rios-Valencia, Diana G., Mompala-Garcia, Yazmin, Marquez-Navarro, Adrian, Tirado-Mendoza, Rocio, Ambrosio, Javier
Publicada:
1 jun 2022
Categoría:
Parasitology
Resumen:
Purpose Annexins are proteins with important roles in parasites, some of
which are related to excretion-secretion processes, protein traffic, and
microvesicle functionality. The participation of annexins in
osmoregulation has been reported in tapeworms, including Taenia solium.
This study aimed to investigate the localization and expression of
annexin in cysticerci of Taenia crassiceps, used as a model of
cysticercosis. Methods We used an antibody made with a protein,
previously employed on Schistosoma bovis, to detect annexin in T.
crassiceps proteins extracts used Western blot assay. The histological
distribution of annexin was studied with immunofluorescence and confocal
microscopy. Results The antibody against annexin recognized a band at a
molecular weight of 40.9 kDa. The histological distribution of annexin
showed that the protein is mainly localized in the tegument and the
protonephridia ducts. Conclusion In our study, annexin was detected at a
molecular weight similar to that described for Schistosoma bovis. In
addition, its principal localization entailed structures of the
osmoregulatory system one of the most important by the survival of the
parasites. This confirms and solidifies previous reports concerning the
role of annexins in T. crassiceps and this will be interesting by the
development of new compounds against this protein.
Filiaciones:
Rios-Valencia, Diana G.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Mompala-Garcia, Yazmin:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Marquez-Navarro, Adrian:
Comis Fed Protecc Riesgos Sanitarios COFEPRIS, Oklahoma 14, Mexico City 03810, DF, Mexico
Tirado-Mendoza, Rocio:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Ambrosio, Javier:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
|