The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis
Por:
Bobes, Raul J., Estrada, Karel, Rios-Valencia, Diana G., Calderon-Gallegos, Arturo, de la Torre, Patricia, Carrero, Julio C., Sanchez-Flores, Alejandro, Laclette, Juan P.
Publicada:
10 may 2022
Resumen:
Human cysticercosis by Taenia solium is the major cause of neurological
illness in countries of Africa, Southeast Asia, and the Americas.
Publication of four cestode genomes (T. solium, Echinococcus
multilocularis, E. granulosus and Hymenolepis microstoma) in the last
decade, marked the advent of novel approaches on the study of the
host-parasite molecular crosstalk for cestode parasites of importance
for human and animal health. Taenia crassiceps is another cestode
parasite, closely related to T. solium, which has been used in numerous
studies as an animal model for human cysticercosis. Therefore,
characterization of the T. crassiceps genome will also contribute to the
understanding of the human infection. Here, we report the genome of T.
crassiceps WFU strain, reconstructed to a noncontiguous finished
resolution and performed a genomic and differential expression
comparison analysis against ORF strain. Both strain genomes were
sequenced using Oxford Nanopore (MinION) and Illumina technologies,
achieving high quality assemblies of about 107 Mb for both strains.
Dotplot comparison between WFU and ORF demonstrated that both genomes
were extremely similar. Additionally, karyotyping results for both
strains failed to demonstrate a difference in chromosome composition.
Therefore, our results strongly support the concept that the absence of
scolex in the ORF strain of T. crassiceps was not the result of a
chromosomal loss as proposed elsewhere. Instead, it appears to be the
result of subtle and extensive differences in the regulation of gene
expression. Analysis of variants between the two strains identified
2,487 sites with changes distributed in 31 of 65 scaffolds. The
differential expression analysis revealed that genes related to
development and morphogenesis in the ORF strain might be involved in the
lack of scolex formation.
Filiaciones:
Univ Nacl Autonoma Mexico, Biomed Res Inst, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Biotechnol Inst, Cuernavaca, Mexico
gold, Green Published, All Open Access, Gold, Green
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