Solid lipid nanoparticles by Venturi tube: preparation, characterization and optimization by Box-Behnken design
Por:
Garcia-Salazar, Gilberto, de la Luz Zambrano-Zaragoza, Maria, Serrano-Mora, Eduardo, Olimpia Mendoza-Diaz, Sandra, Leyva-Gomez, Gerardo, Quintanar-Guerrero, David
Publicada:
3 ago 2021
Resumen:
In this study, a Venturi tube is proposed as an efficient static mixer
incorporated into a continuous recirculation system for obtaining solid
lipid nanoparticles (SLN) of monoolein. The device's operating principle
consists of producing a turbulent flux in the throat of a Venturi tube.
Taking advantage of this effect SLN of monoolein were obtained by rapid
diffusion of the organic phase into the aqueous phase (stabilizer),
causing lipid aggregation on the nanometric particles. The main aim of
the present study was to evaluate the critical factors for obtaining the
SLN of monoolein in order to control the independent variables of this
methodology. A Box-Behnken design was used to study such independent
variables (factors) as injection rate (X1), recirculation rate (X2), and
stabilizer (X3) on the dependent variables; namely, process yield (Y1),
particle size (Y2), polydispersity index (Y3) and zeta potential (Y4).
The optimum operating conditions for preparing SLN were: injection rate,
1.6 mL/min; recirculation rate, 4.2 L/min; and stabilizer concentration,
1.0 w/v, with a value of D = 0.84. The predicted responses of the
particle size were 212.0 nm, with a polydispersity index of 0.21, a zeta
potential of -19.9 mV, and a process yield of 96.0%. Under the same
operating condition, SLN formed with different lipids and stabilizers
were obtained with nanometric size and zeta potential of similar to
-30.0 mV. Results show that the Venturi tube method is an innovative and
versatile technique for preparing SLN of nanometric size with high
process yields through a turbulent flow.
Filiaciones:
Garcia-Salazar, Gilberto:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Lab Invest & Posgrad Tecnol Farmaceut, Cuautitlan, Mexico
de la Luz Zambrano-Zaragoza, Maria:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Lab Proc Transformac Alimentos & Tecnol Emergente, Cuautitlan, Mexico
Serrano-Mora, Eduardo:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Lab Invest & Posgrad Tecnol Farmaceut, Cuautitlan, Mexico
Olimpia Mendoza-Diaz, Sandra:
Univ Autonoma Queretaro, Fac Quim, Dept Invest & Posgrad Alimentos, Queretaro, Mexico
Leyva-Gomez, Gerardo:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Farm, Ciudad Univ, Ciudad De Mexico, Mexico
Quintanar-Guerrero, David:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Lab Invest & Posgrad Tecnol Farmaceut, Cuautitlan, Mexico
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