Agavin induces beneficial microbes in the shrimp microbiota under farming conditions
Por:
Ochoa-Romo, Juan Pablo, Cornejo-Granados, Fernanda, Lopez-Zavala, Alonso A., Viana M.T., Sanchez, Filiberto, Gallardo-Becerra, Luigui, Luque-Villegas, Mirna, Valdez-Lopez, Yesenia, Sotelo-Mundo, Rogerio R., Cota-Huizar, Andres, Lopez-Munguia, Agustin, Ochoa-Leyva, Adrian
Publicada:
16 abr 2022
Categoría:
Multidisciplinary
Resumen:
Prebiotics and probiotics have shown a number of beneficial impacts
preventing diseases in cultured shrimps. Complex soluble carbohydrates
are considered ideal for fostering microbiota biodiversity by
fermentable oligosaccharides, disaccharides, monosaccharides, and
polyols (FODMAPS). Here we evaluated the growth performance and
microbiota composition of the white shrimp Litopenaeus vannamei after
dietary intervention using agavin as a FODMAP prebiotic under farming
conditions. Adult L. vannamei were raised at a shrimp farm and the
effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were
compared to an agavin-free basal diet (BD). After 28 days-trial, the
feed conversion ratio, total feed ingested, and protein efficiency ratio
was significantly improved on animals fed with AG2. At the same time, no
effect on growth performance was observed in AG10. Surprisingly, after
sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial
richness and diversity in the hepatopancreas and intestine was found
only in those animals receiving the AG10 diet, while those receiving the
AG2 diet had a decreased richness and diversity, both diets compared to
the BD. The beta diversity analysis showed a clear significant
microbiota clustering by agavin diets only in the hepatopancreas,
suggesting that agavin supplementation had a more substantial
deterministic effect on the microbiota of hepatopancreas than on the
intestine. We analyzed the literature to search beneficial microbes for
shrimp's health and found sequences for 42 species in our 16S data,
being significantly increased Lactobacillus pentosus, Pseudomonas putida
and Pseudomonas synxantha in the hepatopancreas of the AG10 and
Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the
hepatopancreas of the AG2, both compared to BD. Interestingly, when we
analyzed the abundance of 42 beneficial microbes as a single microbial
community ``meta-community,'' found an increase in their abundance as
agavin concentration increases in the hepatopancreas. In addition, we
also sequenced the DNA of agavin and found 9 of the 42 beneficial
microbes. From those, Lactobacillus lactis and Lactobacillus delbrueckii
were found in shrimps fed with agavin (both AG2 and AG10), and
Lysinibacillus fusiformis in AG10 and they were absent the BD diet,
suggesting these three species could be introduced with the agavin to
the diet. Our work provides evidence that agavin supplementation is
associated with an increase of beneficial microbes for the shrimp
microbiota at farming conditions. Our study provides the first evidence
that a shrimp prebiotic may selectively modify the microbiota in an
organ-dependent effect.
Filiaciones:
Departamento de Microbiología Molecular, Instituto de Biotecnología (IBT), Universidad Nacional Autónoma de México (UNAM), Av. Universidad #2001, Col. Chamilpa, MOR, Cuernavaca, 62210, Mexico
Departamento de Ciencias Químico Biológicas, Universidad de Sonora (UNISON), Blvd., Rosales y Luis Encinas, SON, Hermosillo, 83000, Mexico
Instituto de Investigaciones Oceanológicas, Universidad Autónoma de Baja California (UABC), Km 107 carretera Tijuana/Ensenada, BC, Ensenada, 22860, Mexico
Laboratorio de Estructura Biomolecular, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera Gustavo Enrique Astiazarán Rosas Num. 46, Col. La Victoria, SON, Hermosillo, 83304, Mexico
Camarones El Renacimiento SPR de RI, Justino Rubio No. 26, Col Ejidal, SIN, Higuera de Zaragoza, 81330, Mexico
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, UNAM, Avenida Universidad 2001, Col. Chamilpa, MOR, Cuernavaca, 62420, Mexico
gold, Green Published, All Open Access, Gold, Green
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