Morphology and cyclooxygenase-2 and renin expression in the kidney of young spontaneously hypertensive rats
Por:
Mondragon-Huerta, Carmen G., Bautista-Perez, Rocio, Baiza-Gutman, Luis A., Escobar-Sanchez, Maria L., Del Valle-Mondragon, Leonardo, Gerardo Salas-Garrido, Carlos, Castro-Moreno, Patricia, Ibarra-Barajas, Maximiliano
Publicada:
1 mar 2022
Ahead of Print:
1 nov 2021
Categoría:
Veterinary (miscellaneous)
Resumen:
The kidneys play an important role in blood pressure regulation under
normal and pathological conditions. We examined the histological changes
and expression patterns of cyclooxygenase-2, renin, and (pro)renin
receptor (PRR) in the renal cortex of prehypertensive spontaneously
hypertensive rats (SHRs) and Wistar Kyoto rats (WKYs). Moreover, blood
pressure and plasma urea, creatinine, angiotensin II, and angiotensin
(1-7) levels were measured. The results showed that both strains had
similar blood pressure and plasma urea and creatinine levels. The
glomerular area, basement membrane thickness, collagen fiber content,
and arterial wall thickness were greater in SHRs than in WKYs. By
immunohistochemistry, cyclooxygenase-2 was localized in the macula densa
and renal tubules of both strains. In SHRs, cyclooxygenase-2 was
detected in a larger number of tubules, and the cortical expression of
cyclooxygenase-2 was also increased. In both strains, PRR and renin were
localized in the tubular epithelium and juxtaglomerular cells,
respectively. In SHRs, PRR immunolocalization was increased in the
glomerulus. The cortical expression of immature renin was markedly
increased in SHRs compared to that in WKYs, while renin was
significantly decreased. These changes were associated with higher
plasma angiotensin II levels and lower plasma angiotensin (1-7) levels
in SHRs. The results indicate that the kidneys of SHRs showed
morphological changes and variations in cortical expression patterns of
PRR, cyclooxygenase-2, and renin before the development of hypertension.
Filiaciones:
Mondragon-Huerta, Carmen G.:
Univ Nacl Autonoma Mexico, Tlalnepantla, Estado De Mexic, Mexico
Bautista-Perez, Rocio:
Inst Nacl Cardiol Dr Ignacio Chavez, Ciudad De Mexico, Mexico
Baiza-Gutman, Luis A.:
Univ Nacl Autonoma Mexico, Tlalnepantla, Estado De Mexic, Mexico
Escobar-Sanchez, Maria L.:
Univ Nacl Autonoma Mexico, Ciudad De Mexico, Mexico
Del Valle-Mondragon, Leonardo:
Inst Nacl Cardiol Dr Ignacio Chavez, Ciudad De Mexico, Mexico
Gerardo Salas-Garrido, Carlos:
Univ Nacl Autonoma Mexico, Ciudad De Mexico, Mexico
Castro-Moreno, Patricia:
Univ Nacl Autonoma Mexico, Tlalnepantla, Estado De Mexic, Mexico
Ibarra-Barajas, Maximiliano:
Univ Nacl Autonoma Mexico, Tlalnepantla, Estado De Mexic, Mexico
|