Comparative analysis of differential gene expression indicates divergence in ontogenetic strategies of leaves in two conifer genera
Por:
Webster, Cynthia, Figueroa-Corona, Laura, Mendez-Gonzalez, Ivan David, Alvarez-Soto, Lluvia, Neale, David B., Jaramillo-Correa, Juan Pablo, Wegrzyn, Jill L., Vazquez-Lobo, Alejandra
Publicada:
1 feb 2022
Resumen:
In land plants, heteroblasty broadly refers to a drastic change in
morphology during growth through ontogeny. Juniperus flaccida and Pinus
cembroides are conifers of independent lineages known to exhibit leaf
heteroblasty between the juvenile and adult life stage of development.
Juvenile leaves of P. cembroides develop spirally on the main stem and
appear decurrent, flattened, and needle-like; whereas adult
photosynthetic leaves are triangular or semi-circular needle-like, and
grow in whorls on secondary or tertiary compact dwarf shoots. By
comparison, J. flaccida juvenile leaves are decurrent and needle-like,
and adult leaves are compact, short, and scale-like. Comparative
analyses were performed to evaluate differences in anatomy and gene
expression patterns between developmental phases in both species. RNA
from 12 samples was sequenced and analyzed with available software. They
were assembled de novo from the RNA-Seq reads. Following assembly,
63,741 high-quality transcripts were functionally annotated in P.
cembroides and 69,448 in J. flaccida. Evaluation of the orthologous
groups yielded 4140 shared gene families among the four references
(adult and juvenile from each species). Activities related to cell
division and development were more abundant in juveniles than adults in
P. cembroides, and more abundant in adults than juveniles in J.
flaccida. Overall, there were 509 up-regulated and 81 down-regulated
genes in the juvenile condition of P. cembroides and 14 up-regulated and
22 down-regulated genes in J. flaccida. Gene interaction network
analysis showed evidence of co-expression and co-localization of
up-regulated genes involved in cell wall and cuticle formation,
development, and phenylpropanoid pathway, in juvenile P. cembroides
leaves. Whereas in J. flaccida, differential expression and gene
interaction patterns were detected in genes involved in photosynthesis
and chloroplast biogenesis. Although J. flaccida and P. cembroides both
exhibit leaf heteroblastic development, little overlap was detected, and
unique genes and pathways were highlighted in this study.
Filiaciones:
Webster, Cynthia:
Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, United States
Figueroa-Corona, Laura:
Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Mendez-Gonzalez, Ivan David:
Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, United States
Alvarez-Soto, Lluvia:
Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico
Neale, David B.:
Department of Plant Sciences, University of California, Davis, CA, United States
Jaramillo-Correa, Juan Pablo:
Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Wegrzyn, Jill L.:
Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, United States
Vazquez-Lobo, Alejandra:
Centro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico
Green Published, gold, Green Submitted, All Open Access, Gold, Green
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