Microencapsulation of an Extract of Sechium edule (Jacq.) Sw., with Antineoplastic Activity
Por:
Salazar-Aguilar, Sandra, Ruiz-Posadas, Lucero del Mar, Cadena-Iniguez, Jorge, Soto-Hernandez, Marcos, Santiago-Osorio, Edelmiro, Aguiniga-Sanchez, Itzen, Cisneros-Solano, Victor M., Aguirre-Medina, Juan F., Olivera, alvaro D.
Publicada:
1 ene 2022
Resumen:
Microencapsulation is a technique used in pharmaceuticals as an
administration vehicle. Encapsulating secondary metabolites for
therapeutic purposes has been promoted recently. Microencapsulation
based on chitosan was developed for the methanol extract of cv. Perla
negra (S. edule (Jacq.) Sw.) (Cucurbitaceae) fruits to evaluate its
viability as an administration vehicle and to assess the possible
negative interaction between the extract and chitosan.
Microencapsulation was performed by coacervation, implementing a method
with constant sonication. The microparticles obtained were registered by
means of Scanning Electron Microscopy. The presence of the bioactive in
aqueous medium was recorded for release tests, measuring with
spectrophotometry its concentration as a function of time. The
assessment of the biological effect of the microencapsulated extract was
done on the HeLa cell line and control cells (lymphocytes). Microspheres
with an average size of 20 mu m and a loading capacity of 98% were
obtained. The highest concentration of released extract was 24 mu g
mL(-1) at 23 h. The mainly chitosan-based microspheres did not affect
the antiproliferative activity of the extract of cv. Perla negra and
proved to be a potential vehicle for its therapeutic administration. The
empty microspheres made with chitosan also showed to have an
antiproliferative effect, and those loaded with extract showed cellular
inhibition (statistical IC50) of 8 mu g mL(-1) without affecting the
lymphocytes. Chitosan does not interfere with the biological activity of
the metabolites incorporated into the microspheres since they retain
their inhibitory activity on proliferation in tumor cells, thus
constituting a potential vehicle for the therapeutic administration of
fruit extract.
Filiaciones:
Salazar-Aguilar, Sandra:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Res Unit Cell Differentiat & Canc, Hematopoiesis & Leukemia Lab, Mexico City 09230, DF, Mexico
Ruiz-Posadas, Lucero del Mar:
Colegio Postgrad, Campus Montecillo, Montecillo 56230, Texcoco, Mexico
Cadena-Iniguez, Jorge:
Colegio Postgrad, Campus San Luis Potosi, Salinas Hidalgo 78600, Mexico
Soto-Hernandez, Marcos:
Colegio Postgrad, Campus Montecillo, Montecillo 56230, Texcoco, Mexico
Santiago-Osorio, Edelmiro:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Res Unit Cell Differentiat & Canc, Hematopoiesis & Leukemia Lab, Mexico City 09230, DF, Mexico
Aguiniga-Sanchez, Itzen:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Res Unit Cell Differentiat & Canc, Hematopoiesis & Leukemia Lab, Mexico City 09230, DF, Mexico
Autonoma Mexico, Fac Estudios Super Zaragoza, Sch Med, Dept Biomed Sci, Mexico City 09230, DF, Mexico
Cisneros-Solano, Victor M.:
Univ Autonoma Chapingo, Ctr Reg Univ Oriente, Huatusco 94100, Veracruz, Mexico
Aguirre-Medina, Juan F.:
Univ Autonoma Chiapas, Fac Ciencias Agr, Huehuetan 30660, Mexico
Olivera, alvaro D.:
Ctr Univ Reg Este Univ Republ, Dept Desarrollo Tecnol, Rocha 27000, Uruguay
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