High glucose concentrations induce oxidative stress by inhibiting Nrf2 expression in rat Muller retinal cells in vitro
Por:
Silvestre Albert-Garay, Jesus, Rafael Riesgo-Escovar, Juan, Salceda, Rocio
Publicada:
24 ene 2022
Categoría:
Multidisciplinary
Resumen:
Diabetic retinopathy (DR) is a complication of diabetes. Several studies
have implicated oxidative stress as a fundamental factor in the
progression of the disease. The nuclear factor erythroid-2-related
factor 2 (Nrf2) is one of the main regulators of redox homeostasis. Glia
Muller cells (MC) maintain the structural and functional stability of
the retina. The objective of this study was to evaluate the effect of
high glucose concentrations on reactive oxygen species (ROS) production
and Nrf2 expression levels in rat MC. MC were incubated with normal (NG;
5 mM) or high glucose (HG; 25 mM) for different times. Incubation with
HG increased ROS levels from 12 to 48 h but did not affect cell
viability. However, exposure to 3 h of HG caused a transient decrease
Nrf2 levels. At that time, we also observed a decrease in the mRNA
expression of Nrf2 target genes, glutathione levels, and catalase
activity, all of which increased significantly beyond initial levels
after 48 h of incubation. HG exposure leads to an increase in the p65
subunit of nuclear factor-kappa B (NF-kB) levels, and its target genes.
These results suggest that high glucose concentrations lead to
alteration of the redox regulatory capacity of Nrf2 mediated by NF-kB
regulation.
Filiaciones:
Silvestre Albert-Garay, Jesus:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurodesarrollo & Fisiol, Cdmx 04510, Mexico
Rafael Riesgo-Escovar, Juan:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Campus UNAM Juriquilla, Queretaro 76226, Mexico
Salceda, Rocio:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurodesarrollo & Fisiol, Cdmx 04510, Mexico
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