Molecular identification of a PGRMC-2 receptor in maturing oocytes of the zoonotic nematode parasite Trichinella spiralis
Por:
Morales-Montor, Jorge, Colin-Oviedo, Alvaro, Gonzalez, Gloria M., Palma-Nicolás J.P., Sanchez-Gonzalez, Alejandro, Nava-Castro K.E., Dominguez-Ramirez, Lenin, Garcia-Varela, Martin, Del Río-Araiza V.H., Hernandez-Bello, Romel
Publicada:
1 feb 2022
Resumen:
We previously reported that the Trichinella nematode showed higher
parasite loads in one gender than another, but also the parasite molting
rate decreased when it was cultivated in the presence of progesterone.
In this study we explored the hypothesis that the direct effect of
progesterone on Trichinella spiralis could be mediated by a
steroid-binding parasite protein.
We sequenced, cloned and amplified the Cyt-domain of the progesterone
receptor membrane component-2 of Trichinella spiralis (PGRMC2-Ts).
Furthermore, we expressed the protein and developed an antibody to
perform confocal microscopy and flow cytometry. The expression of the
PGRMC2-Ts protein was exclusively detected at the oocyte and the
parasite's cuticle in cross-sections of the parasite, and this
expression was confirmed by western blot and flow cytometry. Molecular
modeling studies and computer docking for the PGRMC2-Ts protein showed
that it is potentially able to bind to progesterone, estradiol,
testosterone, and dihydrotestosterone with different affinities.
Furthermore, phylogenetic analysis demonstrated that T. spiralis PGRMC2
is related to a steroid-binding protein of another platyhelminth.
Progesterone probably acts upon Trichinella spiralis oocytes by binding
to PGRMC2-Ts. Our data showed that the PGRMC2-Ts protein is present in
the parasite's oocytes, a development step that is crucial for the life
cycle of the parasite. Indeed, this research might have implications in
the field of host-parasite co-evolution and the sex-associated
susceptibility to this infection. In a more practical matter, these
results may contribute to the design of new drugs with anti-parasite
effects.
Filiaciones:
Morales-Montor, Jorge:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico
Colin-Oviedo, Alvaro:
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
Gonzalez, Gloria M.:
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
Palma-Nicolás J.P.:
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
Sanchez-Gonzalez, Alejandro:
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
Nava-Castro K.E.:
Laboratorio de Genotoxicología y Medicina Ambientales, Departamento de Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Mexico
Dominguez-Ramirez, Lenin:
Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Sta. Catarina Mártir. Puebla, Mexico
Garcia-Varela, Martin:
Instituto de Biología, Universidad Nacional Autónoma de México, Mexico
Del Río-Araiza V.H.:
Departamento de Parasitologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, Mexico
Hernandez-Bello, Romel:
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
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