Coupling/Uncoupling Reversibility in Isolated Mitochondria from Saccharomyces cerevisiae
Por:
Morales-Garcia, Lilia, Ricardez-Garcia, Carolina, Castañeda-Tamez P., Chiquete-Felix, Natalia, Uribe-Carvajal, Salvador
Publicada:
1 ene 2021
Resumen:
The yeast Saccharomyces cerevisiae uses fermentation as the preferred pathway to obtain ATP and requires the respiratory chain to re-oxidize the NADH needed for activity of Glyceraldehyde-3phosphate. This process is favored by uncoupling of oxidative phosphorylation (OxPhos), which is at least partially controlled by the mitochondrial unspecific pore (Sc MUC). When mitochondrial ATP synthesis is needed as in the diauxic phase or during mating, a large rise in Ca2+ concentration ([Ca2+ ]) closesSc MUC, coupling OxPhos. In addition,Sc MUC opening/closing is mediated by the ATP/ADP ratio, which indicates cellular energy needs. Here, opening and closing ofSc MUC was evaluated in isolated mitochondria from S. cerevisiae at different incubation times and in the presence of different ATP/ADP ratios or varying [Ca2+ ]. Measurements of the rate of O2 consumption, mitochondrial swelling, transmembrane potential and ROS generation were conducted. It was observed thatSc MUC opening was reversible, a high ATP/ADP ratio promoted opening and [Ca2+ ] closedSc MUC even after several minutes of incubation in the open state. In the absence of ATP synthesis, closure of ScMUC resulted in an increase in ROS. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Morales-Garcia, Lilia:
Department of Genetics and Molecular Biology, Instituto de Fisiología Celular, UNAM, Mexico City, 04510, Mexico
Department of Biochemistry, Medicine School, UNAM, Mexico City, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet & Mol Biol, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Sch Med, Dept Biochem, Mexico City 04510, DF, Mexico
Ricardez-Garcia, Carolina:
Department of Genetics and Molecular Biology, Instituto de Fisiología Celular, UNAM, Mexico City, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet & Mol Biol, Mexico City 04510, DF, Mexico
Castañeda-Tamez P.:
Department of Genetics and Molecular Biology, Instituto de Fisiología Celular, UNAM, Mexico City, 04510, Mexico
Chiquete-Felix, Natalia:
Department of Genetics and Molecular Biology, Instituto de Fisiología Celular, UNAM, Mexico City, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet & Mol Biol, Mexico City 04510, DF, Mexico
Uribe-Carvajal, Salvador:
Department of Genetics and Molecular Biology, Instituto de Fisiología Celular, UNAM, Mexico City, 04510, Mexico
Department of Biochemistry, Medicine School, UNAM, Mexico City, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet & Mol Biol, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Sch Med, Dept Biochem, Mexico City 04510, DF, Mexico
gold, Green Published, All Open Access, Gold, Green
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