Characterization of commensal Escherichia coli isolates from slaughtered sheep in Mexico
Por:
Acosta-Dibarrat, Jorge, Enriquez-Gomez, Edgar, Talavera-Rojas, Martin, Soriano-Vargas, Edgardo, Navarro, Armando, Morales-Espinosa, Rosario
Publicada:
1 ene 2021
Resumen:
Introduction: Commensal Escherichia coli is defined as bacteria without known virulence factors that could be playing a specific role in some diseases; however, they could be responsible to disseminate antimicrobial resistance genes to other microorganisms. This study aimed to characterize the commensal E. coli isolates obtained from slaughtered sheep in the central region of Mexico. Methodology: Isolates were classified as commensal E. coli when distinctive genes related to diarrheagenic pathotypes (stx1, stx2, eae, bfp, LT, stp, ipaH, and aggR) were discarded by PCR. Identification of serotype, phylogenetic group, and antimicrobial resistance was also performed. Results: A total of 41 isolates were characterized. The phylogenetic groups found were B1 in 37 isolates (90.2%), A in 2 (4.8%), and 1 isolate (2.4%) for C and D groups. Serotypes associated with diarrhea in humans (O104:H2 and O154:NM) and hemolytic uremic syndrome (O8:NM) were detected. Thirty-three isolates (80%) were resistant to ceftazidime, 23 (56%), to tetracycline 8 (19.5%) to ampicillin, and 1 to amikacin. Six isolates (14.6%) were multidrug-resistant. Conclusions: This study provides new information about commensal E. coli in slaughtered sheep, high percentages of resistance to antibiotics, and different profiles of antimicrobial resistance were found, their dissemination constitute a risk factor towards the consuming population. Copyright © 2021 Acosta-Dibarrat et al. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Filiaciones:
Acosta-Dibarrat, Jorge:
Center for Research and Advanced Studies in Animal Health, Faculty of Veterinary Medicine and Zootechnics, Universidad Autónoma del Estado de México, Estado de México, Toluca, Mexico
Enriquez-Gomez, Edgar:
Center for Research and Advanced Studies in Animal Health, Faculty of Veterinary Medicine and Zootechnics, Universidad Autónoma del Estado de México, Estado de México, Toluca, Mexico
Talavera-Rojas, Martin:
Center for Research and Advanced Studies in Animal Health, Faculty of Veterinary Medicine and Zootechnics, Universidad Autónoma del Estado de México, Estado de México, Toluca, Mexico
Soriano-Vargas, Edgardo:
Center for Research and Advanced Studies in Animal Health, Faculty of Veterinary Medicine and Zootechnics, Universidad Autónoma del Estado de México, Estado de México, Toluca, Mexico
Navarro, Armando:
Department of Public Health, Faculty of Medicine, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Colonia Copilco, Delegación Coyoacan, México City, Mexico
Morales-Espinosa, Rosario:
Laboratory of Bacterial Genomics, Department of Microbiology and Parasitology, Faculty of Medicine, Universidad Nacional Autónoma de México, Colonia Copilco, México City, Mexico
gold, All Open Access; Gold
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