Ozone as an alternative decontamination process for N95 facemask and biosafety gowns
Por:
Ibanez-Cervantes, G., Lugo-Zamudio G.E., Cruz-Cruz C., Durán-Manuel E.M., Bravata-Alcántara J.C., García-Moncada E., Mata-Rocha M., Delgado-Balbuena L., Cureno-Diaz, M. A., Ramírez-Cortina C.R., León-Ávila G., Nogueda-Torres B., Hernández-Hernández J.M., Rodil S.E., Bello-López J.M.
Publicada:
15 mar 2022
Resumen:
COVID-19 pandemic created a global shortage of medical protective
equipment. Here, we considered ozone (O3) a disinfectant alternative due
to its potent oxidative activity against biological macromolecules. The
O3 decontamination assays were done using SARS-CoV-2 obtained from
patients to produce artificial contamination of N95 masks and
biosecurity gowns. The quantification of SARS-CoV-2 was performed before
and after exposing the samples to different ozone gas concentrations for
times between 5 and 30 min. Viral loads as a function of the O3 exposure
time were estimated from the data obtained by the RT-PCR technique. The
genetic material of the virus was no longer detected for any tested
concentrations after 15 min of O3 exposure, which means a disinfection
Concentration-Time above 144 ppm min. Vibrational spectroscopies were
used to follow the modifications of the polymeric fibers after the O3
treatment. The results indicate that the N95 masks could be safely
reused after decontamination with treatments of 15 min at the
established O3 doses for a maximum of 6 cycles.
Filiaciones:
Ibanez-Cervantes, G.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Inst Politecn Nacl, Escuela Super Med, Ciudad De Mexico, Mexico
Lugo-Zamudio G.E.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Cruz-Cruz C.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Durán-Manuel E.M.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Bravata-Alcántara J.C.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
García-Moncada E.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Mata-Rocha M.:
Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Hosp Pediat, Ciudad De Mexico, Mexico
Delgado-Balbuena L.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Cureno-Diaz, M. A.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Ramírez-Cortina C.R.:
Univ Autonoma Metropolitana, Dept Energia, Ciudad De Mexico, Mexico
León-Ávila G.:
IPN, Escuela Nacl Ciencias Biol, Dept Zool, Ciudad De Mexico, Mexico
Nogueda-Torres B.:
IPN, Escuela Nacl Ciencias Biol, Dept Parasitol, Ciudad De Mexico, Mexico
Hernández-Hernández J.M.:
IPN, Ctr Invest & Estudios Avanzados, Dept Biol Celular, Ciudad De Mexico, Mexico
Rodil S.E.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Ciudad De Mexico, Mexico
Bello-López J.M.:
Hosp Juarez Mexico, Ciudad De Mexico, Mexico
Green Accepted, Green
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