Variability in Behavioral Phenotypes after Forced Swimming-Induced Stress in Rats Is Associated with Expression of the Glucocorticoid Receptor, Nurr1, and IL-1 beta in the Hippocampus
Por:
Ruiz-Sanchez, Elizabeth, Lopez-Ramirez, Arely M., Ruiz-Chow, Angel, Calvillo, Minerva, Resendiz-Albor, Aldo A., Anguiano, Brenda, Rojas, Patricia
Publicada:
1 dic 2021
Resumen:
Individual differences in coping with stress may determine either a
vulnerable or resilient phenotype. Therefore, it is important to better
understand the biology underlying the behavioral phenotype. We assessed
whether individual behavioral phenotype to acute stress is related with
the hippocampal expression of glucocorticoid receptor (GR), Nurr1,
interleukin-1 beta (IL-1 beta) or brain-derived neurotrophic factor
(BDNF). Wistar male rats were exposed to forced swimming for 15 min and
sacrificed at different times. Behavioral response was analyzed, and it
was compared with the gene and protein expression of GR, Nurr1, IL-1
beta and BDNF in the hippocampus for each time point. Behavioral
phenotyping showed a group with high immobility (vulnerable) while
another had low immobility (resilient). No significant differences were
found in the Nurr1, IL-1 beta and BDNF mRNA levels between resilient and
vulnerable rats at different recovery times except for Nr3c1 (gene for
GR). However, exposure to stress caused significantly higher levels of
GR, Nurr1 and IL-1 beta proteins of vulnerable compared to resilient
rats. This variability of behavioral phenotypes is associated with a
differential molecular response to stress that involves GR, Nurr1, and
IL-1 beta as mediators in coping with stress. This contributes to
identifying biomarkers of susceptibility to stress.
Filiaciones:
Ruiz-Sanchez, Elizabeth:
Laboratory of Neurotoxicology, National Institute of Neurology and Neurosurgery Manuel Velasco Suárez, SS, Av. Insurgentes Sur No. 3877, Mexico City, C.P. 14269, Mexico
Lopez-Ramirez, Arely M.:
Laboratory of Neurotoxicology, National Institute of Neurology and Neurosurgery Manuel Velasco Suárez, SS, Av. Insurgentes Sur No. 3877, Mexico City, C.P. 14269, Mexico
Ruiz-Chow, Angel:
Neuropsychiatry Unit, National Institute of Neurology and Neurosurgery Manuel Velasco Suárez, SS, Av. Insurgentes Sur No. 3877, Mexico City, C.P. 14269, Mexico
Calvillo, Minerva:
Experimental Laboratory of Neurodegenerative Diseases, National Institute of Neurology and Neurosurgery Manuel Velasco Suárez, SS, Av. Insurgentes Sur No. 3877, Mexico City, C.P. 14269, Mexico
Resendiz-Albor, Aldo A.:
Mucosal Immunity Laboratory, Research and Graduate Section, Instituto Politécnico Nacional, Escuela Superior de Medicina, Plan de San Luis Esq. Salvador Díaz Mirón s/n, Mexico City, C.P. 11340, Mexico
Anguiano, Brenda:
Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM), Campus Juriquilla, Boulevard Juriquilla 3001, Querétaro, C.P. 76230, Mexico
Rojas, Patricia:
Laboratory of Neurotoxicology, National Institute of Neurology and Neurosurgery Manuel Velasco Suárez, SS, Av. Insurgentes Sur No. 3877, Mexico City, C.P. 14269, Mexico
Green Published, gold, Gold
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