Comprehensive profiling and identification of bioactive components from methanolic leaves extract of juniperus deppeana and its in vitro antidiabetic activity
Por:
Monribot-Villanueva J.L., Rodríguez-Fuentes J.S., Landa-Cansigno C., Infante-Rodríguez D.A., Díaz-Abad J.P., Guerrero-Analco J.A.
Publicada:
1 ene 2020
Resumen:
Juniperus plant species are rich sources of bioactive secondary metabolites and are traditionally used for the treatment of several illnesses, including those related to hyperglycemia and diabetes. The major bioactive compounds identified in certain species of this genus are terpenes and phenolics. Juniperus deppeana Steud. is mainly used as a wood resource and its chemical composition has been partially established. Our goal was to perform a comprehensive profiling of a methanolic extract of leaves of J. deppeana and determine its potential as a source of a-amylase and a-glucosidase inhibitors. Terpene and phenolic compounds were putatively identified based on their accurate mass spectrometric data. Regarding terpenes, we found mainly diterpenes, specifically dehydroabietic acid-like, hinokiol-like, agathic acid-like, and dihydroxyabietatrienoic acid-like compounds. Isopimaric acid was also identified and its identity was confirmed by coelution with an authentic standard via comparing retention time, mass spectrum, and collisional cross section values. For phenolic compounds, we identified mainly compounds with a chemical structure similar to the biflavonoids amentoflavone and bilobetin. Besides, the methanolic extract of J. deppeana leaves show inhibition of a-amylase (IC50 = 85.11 ± 11.91 µg mL-1) and a-glucosidase (IC50 = 32.50 ± 3.40 µg mL-1) enzymes, demonstrating a potential alternative for the search of antidiabetic natural products. © 2020, Canadian Science Publishing. All rights reserved.
Filiaciones:
Monribot-Villanueva J.L.:
Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimi®, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91073, Mexico
Rodríguez-Fuentes J.S.:
Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimi®, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91073, Mexico
Landa-Cansigno C.:
Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimi®, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91073, Mexico
Infante-Rodríguez D.A.:
Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimi®, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91073, Mexico
Díaz-Abad J.P.:
Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimi®, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91073, Mexico
Guerrero-Analco J.A.:
Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimi®, Instituto de Ecología A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91073, Mexico
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