Differential activity on trypanosomatid parasites of a novel recombinant defensin type 1 from the insect Triatoma (Meccus) pallidipennis
Por:
Diaz-Garrido, Paulina, Cardenas-Guerra, Rosa Elena, Martinez, Ignacio, POGGIO, SEBASTIAN, Rodriguez-Hernandez, Karla, Rivera-Santiago, Lucio, Ortega-Lopez, Jaime, Sanchez-Esquivel, Sergio, Espinoza, Bertha
Publicada:
1 dic 2021
Resumen:
Defensins are one of the major families of antimicrobial peptides (AMPs)
that are widely distributed in insects. In Triatomines (Hemiptera:
Reduviidae) vectors of Trypanosoma cruzi the causative agent of Chagas
disease, two large groups of defensin isoforms have been described: type
1 and type 4. The aim of this study was to analyze the trypanocidal
activity of a type 1 recombinant defensin (rDef1.3) identified in
Triatoma (Meccus) pallidipennis, an endemic specie from Me ` xico. The
trypanocidal activity of this defensin was evaluated in vitro, against
the parasites T. cruzi, T. rangeli, and two species of Leishmania (L.
mexicana and L. major) both causative agents of cutaneous leishmaniasis.
Our data demonstrated that the defensin was active against all the
parasites although in different degrees. The defensin altered the
morphology, reduced the viability and inhibited the growth of T. cruzi.
When tested against T. rangeli (a parasite that infects a variety of
mammalian species), stronger morphological effects where observed.
Surprisingly the greatest effects were observed against the two
Leishmania species, of which L. major was the parasite most affected
with 50% of dead cells or with damaged membranes, in addition of a
reduction in its proliferative capacity in culture. These results
suggest that rDef1.3 has an important antimicrobial effect against
trypanosomatids which cause some of the more important neglected
tropical diseases transmitted by insect vectors.
Filiaciones:
Diaz-Garrido, Paulina:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Lab Estudios Tripanosomiasis, Dept Inmunol, Mexico City 04510, DF, Mexico
Cardenas-Guerra, Rosa Elena:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Lab Estudios Tripanosomiasis, Dept Inmunol, Mexico City 04510, DF, Mexico
Martinez, Ignacio:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Lab Estudios Tripanosomiasis, Dept Inmunol, Mexico City 04510, DF, Mexico
POGGIO, SEBASTIAN:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Mexico City 04510, DF, Mexico
Rodriguez-Hernandez, Karla:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Lab Estudios Tripanosomiasis, Dept Inmunol, Mexico City 04510, DF, Mexico
Rivera-Santiago, Lucio:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Lab Estudios Tripanosomiasis, Dept Inmunol, Mexico City 04510, DF, Mexico
Ortega-Lopez, Jaime:
Ctr Invest Estudios Avanzados Inst Politecn Nacl, Dept Biotecnol & Bioingn, Av IPN 2508,Col San Pedro Zacatenco, Mexico City 07360, DF, Mexico
Sanchez-Esquivel, Sergio:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Mexico City 04510, DF, Mexico
Espinoza, Bertha:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Lab Estudios Tripanosomiasis, Dept Inmunol, Mexico City 04510, DF, Mexico
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