Biocide effect against SARS-CoV-2 and ESKAPE pathogens of a noncytotoxic silver-copper nanofilm
Por:
Bello-Lopez, J. M., Silva-Bermudez, P., Prado, G., Martinez, A., Ibanez-Cervantes, Gabriela, Cureno-Diaz, Monica Alethia, Rocha-Zavaleta, L., Manzo-Merino, J., Almaguer-Flores, A., Ramos-Vilchis, C., Rodil, S. E.
Publicada:
1 ene 2022
Resumen:
Nanometric materials with biocidal properties effective against severe
acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) and pathogenic
bacteria could be used to modify surfaces, reducing the risk of touching
transmission. In this work, we showed that a nanometric layer of
bimetallic AgCu can be effectively deposited on polypropylene (PP)
fibers. The virucidal properties of the AgCu nanofilm were evaluated by
comparing the viral loads remaining on uncoated and coated PP after
contact times between 2 and 24 h. Quantification of virion numbers for
different initial concentrations indicated a reduction of more than 95%
after 2 h of contact. The bactericidal action of the AgCu nanofilm was
also confirmed by inoculating uncoated and coated PP with a pool of
pathogenic bacteria associated with pneumonia (ESKAPE). Meanwhile, no
cytotoxicity was observed for human fibroblasts and keratinocyte cells,
indicating that the nanofilm could be in contact with human skin without
threat. The deposition of the AgCu nanofilm on the nonwoven component of
reusable cloth masks might help to prevent virus and bacterial infection
while reducing the pollution burden related to the disposable masks. The
possible mechanism of biocide contact action was studied by quantum
chemistry calculations that show that the addition of Ag and/or Cu makes
the polymeric fiber a better electron acceptor. This can promote the
oxidation of the phospholipids present at both the virus and bacterial
membranes. The rupture at the membrane exposes and damages the genetic
material of the virus. More studies are needed to determine the
mechanism of action, but the results reported here indicate that Cu and
Ag ions are good allies, which can help protect us from the virus that
has caused this disturbing pandemic.
Filiaciones:
Bello-Lopez, J. M.:
Hosp Juarez Mexico, Direcc Invest, Magdalena Salinas, Av Inst Politecn Nacl 5160, Cdmx 07760, Mexico
Silva-Bermudez, P.:
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarr, Unidad Ingn Tejidos Terapia Celular & Med Regener, Av Mexico Xochimilco 289 Col Arenal Guadalupe, Cdmx 14389, Mexico
Prado, G.:
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Lab Biotecnol, Av Mexico Xochimilco 289 Col Arenal Guadalupe, Cdmx 14389, Mexico
Ibanez-Cervantes, Gabriela:
Hosp Juarez Mexico, Direcc Invest, Magdalena Salinas, Av Inst Politecn Nacl 5160, Cdmx 07760, Mexico
Cureno-Diaz, Monica Alethia:
Hosp Juarez Mexico, Direcc Invest, Magdalena Salinas, Av Inst Politecn Nacl 5160, Cdmx 07760, Mexico
Rocha-Zavaleta, L.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Circuito Escolar Sn,Ciudad Univ, Cdmx 04510, Mexico
Manzo-Merino, J.:
Catedras CONACyT, Inst Nacl Cancerol, Cdmx, Mexico
Almaguer-Flores, A.:
Univ Nacl Autonoma Mexico, Lab Biointerfases, Fac Odontol, Div Estudios Posgrado & Invest, Cdmx 04510, Mexico
Ramos-Vilchis, C.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Circuito Exterior Sn,Ciudad Univ, Cdmx 04510, Mexico
Rodil, S. E.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Circuito Exterior Sn,Ciudad Univ, Cdmx 04510, Mexico
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