Integrative taxonomy reveals an even greater diversity within the speciose genus Phyllodistomum (Platyhelminthes : Trematoda : Gorgoderidae), parasitic in the urinary bladder of Middle American freshwater fishes, with descriptions of five new species
Por:
Pinacho-Pinacho, Carlos D., Sereno-Uribe, Ana L., Orts, Jesus S. Hernandez, Garcia-Varela, Martin, Perez-Ponce de Leon, Gerardo
Publicada:
1 sep 2021
Ahead of Print:
1 sep 2021
Categoría:
Ecology, evolution, behavior and systematics
Resumen:
Phyllodistomum is one of the most species-rich genera of parasitic
platyhelminths, with 120 species described worldwide; they infect the
urinary bladder of marine and freshwater fishes. As the number of new
species within the genus has increased, morphological conservatism, and
the lack of reliable diagnostic traits make the separation of species a
challenging task. The increase of genetic data for Phyllodistomum
species has permitted the use of an integrative taxonomy approach as a
framework for species discovery and delimitation. DNA sequences (28S
rRNA and COI mtDNA) were obtained from individuals of Phyllodistomum
sampled in 29 locations across Middle America, and used in combination
with morphology, host association and geographic distribution to uncover
five new congeneric species. Morphologically, the new species are
relatively similar; there are no unique morphological traits to readily
distinguish them. We first investigated species boundaries through
phylogenetic analyses of the independent and concatenated datasets;
analyses recognised five candidate species showing reciprocal monophyly
and strong clade support, particularly for COI data. The interspecific
28S rRNA and COI sequence divergence among the new species from 0.4 to
18.4% and from 5.1 to 27% respectively. These results were further
validated by a Bayesian species delimitation approach. The five new
species are well supported by molecular data used in combination with
other sources of information such as host association and geographical
distribution and are described herein as Phyllodistomum romualdae sp.
nov., P. virmantasi sp. nov., P. isabelae sp. nov., P. scotti sp. nov.,
and P. simonae sp. nov.
Filiaciones:
Pinacho-Pinacho, Carlos D.:
Inst Ecol AC, Catedras CONACyT, Red Estudios Mol Avanzados, Kilometro 2-5 Ant Carretera Coatepec, Xalapa 91070, Veracruz, Mexico
Sereno-Uribe, Ana L.:
Univ Nacl Autonoma Mexico, Inst Biol, Apartadod Postal 70-153,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Orts, Jesus S. Hernandez:
Czech Acad Sci, Biol Ctr, Inst Parasitol, Branisovska 31, Ceske Budejovice 37005, Czech Republic
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Inst Biol, Apartadod Postal 70-153,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Perez-Ponce de Leon, Gerardo:
Univ Nacl Autonoma Mexico, Inst Biol, Apartadod Postal 70-153,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Merida ENES Merida, Kilometro 4-5 Carretera Merida Tetiz, Municipio De Ucu 97357, Yucatan, Mexico
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