Identification and functional characterization of the siRNA pathway in Taenia crassiceps by silencing Enolase A
Por:
Guerrero-Hernandez, Julio, Bobes, Raul J., Garcia-Varela, Martin, Castellanos-Gonzalez, Alejandro, Laclette, Juan P.
Publicada:
1 ene 2022
Resumen:
A gene silencing procedure on cysticerci of the taeniid cestode Taenia crassiceps is described. This is the first time this technique is reported in this species that is widely used as an animal model for human cysticercosis. Genome database searches were performed in order to find out if relevant genes involved in gene silencing and non-coding RNA processing, Argonaute and Dicer (AGO and Dcr) are present in T. crassiceps. We found three AGO and two Dcr orthologues that were designed TcAGO1, Tc2 and Tc3, as well as TcDcr1 and TcDcr2. In order to elucidate the evolutionary relationships of T. crassiceps TcAGO and TcDcr genes, separate phylogenetic analyses were carried out for each, including AGO and Dcr orthologues of other 20 platyhelminthes. Our findings showed a close phylogenetic relationship of TcAGO and TcDcr with those previously described for Echinococcus spp. Our RT-PCR studies demonstrated expression of all TcAGO and TcDcr orthologues. Our results show that the gene silencing machinery in T. crassiceps is functionally active by inducing silencing of TcEnoA (~90%). These results clearly show that gene silencing using siRNAs can be used as a molecular methodology to study gene function in taeniid cestodes. © 2021
Filiaciones:
Guerrero-Hernandez, Julio:
Biomedical Research Institute. Universidad Nacional Autónoma de México, México, Coyoacán, Cd. de México 04510, Mexico
Univ Nacl Autonoma Mexico, Biomed Res Inst, Cd De Mexico 04510, Mexico
Bobes, Raul J.:
Biomedical Research Institute. Universidad Nacional Autónoma de México, México, Coyoacán, Cd. de México 04510, Mexico
Univ Nacl Autonoma Mexico, Biomed Res Inst, Cd De Mexico 04510, Mexico
Garcia-Varela, Martin:
Biological Institute. Universidad Nacional Autónoma de México, Coyoacán, Cd. de México 04510, Mexico
Univ Nacl Autonoma Mexico, Biol Inst, Cd De Mexico 04510, Mexico
Castellanos-Gonzalez, Alejandro:
Division of Infectious Diseases, University of Texas Medical Branch, United States
Center for Tropical Diseases, University of Texas Medical Branch, United States
Univ Texas Med Branch, Div Infect Dis, 301 Univ, Galveston, TX 77555 USA
Univ Texas Med Branch, Ctr Trop Dis, Galveston, TX 77555 USA
Laclette, Juan P.:
Biomedical Research Institute. Universidad Nacional Autónoma de México, México, Coyoacán, Cd. de México 04510, Mexico
Univ Nacl Autonoma Mexico, Biomed Res Inst, Cd De Mexico 04510, Mexico
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