Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico
Por:
Flores, Yvonne N., Amoon, Aryana T., Su, Baolong, Velazquez-Cruz, Rafael, Ramirez-Palacios, Paula, Salmeron, Jorge, Rivera-Paredez, Berenice, Sinsheimer, Janet S., Lusis, Aldons J., Huertas-Vazquez, Adriana, Saab, Sammy, Glenn, Beth A., May, Folasade P., Williams, Kevin J., Bastani, Roshan, Bensinger, Steven J.
Publicada:
10 oct 2021
Resumen:
Background: Nonalcoholic fatty liver disease (NAFLD) is a leading cause
of chronic liver disease and cirrhosis. NAFLD is mediated by changes in
lipid metabolism and known risk factors include obesity, metabolic
syndrome, and diabetes. The aim of this study was to better understand
differences in the lipid composition of individuals with NAFLD compared
to controls, by performing direct infusion lipidomics on serum
biospecimens from a cohort study of adults in Mexico.
Methods: A nested case-control study was conducted with a sample of 98
NAFLD cases and 100 healthy controls who are participating in an
on-going, longitudinal study in Mexico. NAFLD cases were clinically
confirmed using elevated liver enzyme tests and liver ultrasound or
liver ultrasound elastography, after excluding alcohol abuse, and 100
controls were identified as having at least two consecutive normal
alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (<
40 U/L) results in a 6-month period, and a normal liver ultrasound
elastography result in January 2018. Samples were analyzed on the Sciex
Lipidyzer Platform and quantified with normalization to serum volume. As
many as 1100 lipid species can be identified using the Lipidyzer
targeted multiple-reaction monitoring list. The association between
serum lipids and NAFLD was investigated using analysis of covariance,
random forest analysis, and by generating receiver operator
characteristic (ROC) curves.
Results: NAFLD cases had differences in total amounts of serum
cholesterol esters, lysophosphatidylcholines, sphingomyelins, and
triacylglycerols (TAGs), however, other lipid subclasses were similar to
controls. Analysis of individual TAG species revealed increased
incorporation of saturated fatty acyl tails in serum of NAFLD cases.
After adjusting for age, sex, body mass index, and PNPLA3 genotype, a
combined panel of ten lipids predicted case or control status better
than an area under the ROC curve of 0.83.
Conclusions: These preliminary results indicate that the serum lipidome
differs in patients with NAFLD, compared to healthy controls, and
suggest that assessing the desaturation state of TAGs or a specific
lipid panel may be useful clinical tools for the diagnosis of NAFLD.
Filiaciones:
Univ Calif Los Angeles, Fielding Sch Publ Hlth, Dept Hlth Policy & Management, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, Ctr Canc Prevent & Control, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, Kaiser Permanente Ctr Hlth Equ, Fielding Sch Publ Hlth, Los Angeles, CA 90095 USA
Jonsson Comprehens Canc Ctr, Los Angeles, CA 90034 USA
Inst Mexicano Seguro Social, Unidad Invest Epidemiol & Serv Salud, Cuernavaca, Morelos, Mexico
Univ Calif Los Angeles, David Geffen Sch Med, Lipid Lab, Los Angeles, CA 90095 USA
Inst Nacl Med Genom INMEGEN, Lab Genom Metab Oseo, Ciudad De Mexico, Mexico
Univ Nacl Autonoma Mexico, Ctr Invest Polit Poblac & Salud, Ciudad De Mexico, Mexico
Univ Calif Los Angeles, Dept Human Genet & Computat Med, Los Angeles, CA USA
Univ Calif Los Angeles, Dept Biostat, Fielding Sch Publ Hlth, Los Angeles, CA USA
Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Cardiol, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, David Geffen Sch Med, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Vatche & Tamar Manoukian Div Digest Dis, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, David Geffen Sch Med, Pfleger Liver Inst, Los Angeles, CA 90095 USA
VA Greater Los Angeles Healthcare Syst, Dept Med, Los Angeles, CA USA
Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA
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