Anthelmintic-like Activity of Leucaena leucocephala Aqueous Extract against Gyrodactylus spp. in Naturally Infected Tilapia Fingerlings
Por:
Compean-Martinez, Juan, Salazar-Ulloa, Martha, Chavez-Soriano, Larisa, Munoz-Cordoba, German, von Son-de Fernex, Elke
Publicada:
1 oct 2021
Ahead of Print:
1 ago 2021
Categoría:
Aquatic science
Resumen:
Aquaculture represents the fastest growing food-producing sector
worldwide. However, the intensification of fish farms has led to
environmental, health, and welfare challenges. The monogenean
ectoparasite Gyrodactylus spp. has been considered one of the most
pathogenic ectoparasites affecting fish, and no specific control
strategies besides formalin baths have been established. The use of
bioactive plants has been recently proposed as an alternative method to
control gyrodactylid infections. Leucaena leucocephala is a tropical
legume that has been found with anthelmintic activity against both
internal and external parasites of cattle. Therefore, the objective of
this study was to assess the anthelmintic-like activity of L.
leucocephala aqueous extract against Gyrodactylus spp. in hybrid tilapia
Oreochromis spp. fingerlings and its effect on host health through
histopathological analysis. Five concentrations of L. leucocephala
aqueous extract were employed (2.00, 1.00, 0.50, 0.25, and 0.125 mg/mL)
to estimate the mean effective concentration (EC50). Distilled water and
formalin (1:4,000) were used as negative and positive controls,
respectively. The aqueous extract of L. leucocephala showed a
dose-dependent behavior, with an EC50 of 0.228 mg/mL. Quercetin was
identified as the main phytochemical present in the extract. Postmortem
analysis of fingerlings exposed to the plant extract had no
treatment-associated pathological findings, thus indicating that the use
of L. leucocephala baths had no negative impact that could jeopardize
the lives of treated animals. After the in vivo assessment, the aqueous
extract of L. leucocephala appears to be a promising alternative to
control gyrodactylid infection in tilapia.
Filiaciones:
Univ Autonoma Zacatecas, Jardin Juarez 147, Zacatecas 98000, Zacatecas, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Ctr Ensenanza Invest & Extens Ganaderia Trop, Km 5-5 Carretera Fed, Tlapacoyan Martinez Torr 93600, Veracruz, Mexico
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Patol, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
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