Novel findings to the biosynthetic pathway of magnoflorine and taspine through transcriptomic and metabolomic analysis of Croton draco (Euphorbiaceae)
Por:
Canedo-Téxon A., Ramón-Farias F., Monribot-Villanueva J.L., Villafán E., Alonso-Sánchez A., Pérez-Torres C.A., Ángeles G., Guerrero-Analco J.A., Ibarra-Laclette E.
Publicada:
1 ene 2019
Categoría:
Plant science
Resumen:
Background: Croton draco is an arboreal species and its latex as well as some other parts of the plant, are traditionally used in the treatment of a wide range of ailments and diseases. Alkaloids, such as magnoflorine, prevent early atherosclerosis progression while taspine, an abundant constituent of latex, has been described as a wound-healer and antitumor-agent. Despite the great interest for these and other secondary metabolites, no omics resources existed for the species and the biosynthetic pathways of these alkaloids remain largely unknown. Results: To gain insights into the pathways involved in magnoflorine and taspine biosynthesis by C. draco and identify the key enzymes in these processes, we performed an integrated analysis of the transcriptome and metabolome in the major organs (roots, stem, leaves, inflorescences, and flowers) of this species. Transcript profiles were generated through high-throughput RNA-sequencing analysis while targeted and high resolution untargeted metabolomic profiling was also performed. The biosynthesis of these compounds appears to occur in the plant organs examined, but intermediaries may be translocated from the cells in which they are produced to other cells in which they accumulate. Conclusions: Our results provide a framework to better understand magnoflorine and taspine biosynthesis in C. draco. In addition, we demonstrate the potential of multi-omics approaches to identify candidate genes involved in the biosynthetic pathways of interest. © 2019 The Author(s).
Filiaciones:
Canedo-Téxon A.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
Ramón-Farias F.:
Universidad Veracruzana (Campus Peñuela-Córdoba), Amatlán de Los Reyes, Veracruz, 94945, Mexico
Monribot-Villanueva J.L.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
Villafán E.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
Alonso-Sánchez A.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
Pérez-Torres C.A.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
Catedrático CONACyT en El Instituto de Ecología A.C, Veracruz, Mexico
Ángeles G.:
Instituto de Ecología A.C., Red de Ecología Funcional, Xalapa, Veracruz, 91070, Mexico
Guerrero-Analco J.A.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
Ibarra-Laclette E.:
Instituto de Ecología A.C., Red de Estudios Moleculares Avanzados, Xalapa, Veracruz, 91070, Mexico
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