Exposure to Parasitic Protists and Helminths Changes the Intestinal Community Structure of Bacterial Communities in a Cohort of Mother-Child Binomials from a Semirural Setting in Mexico
Por:
Partida-Rodriguez, Oswaldo, Nieves-Ramirez, Miriam, Laforest-Lapointe, Isabelle, Brown, Eric M., Parfrey, Laura, Valadez-Salazar, Alicia, Thorson, Lisa, Moran, Patricia, Gonzalez, Enrique, Rascon, Edgar, Magaña U., Hernandez, Eric, Rojas-Velazquez, Liliana, Torres, Javier, Arrieta, Marie Claire, Ximenez, Cecilia, Finlay, B. Brett
Publicada:
1 jul 2021
Resumen:
An estimated 3.5 billion people are colonized by intestinal parasites
worldwide. Intestinal parasitic eukaryotes interact not only with the
host but also with the intestinal microbiota. In this work, we studied
the relationship between the presence of multiple enteric parasites and
the community structures of gut bacteria and eukaryotes in an
asymptomatic mother-child cohort from a semirural community in Mexico.
Fecal samples were collected from 46 mothers and their respective
children, with ages ranging from 2 to 20 months. Mothers and infants
were found to be multiparasitized by Blastocystis hominis, Entamoeba
dispar, Endolimax nana, Chilomastix mesnili, lodamoeba butshlii,
Entamoeba coil, Hymenolepis nana, and Ascaris lumbricoides. Sequencing
of bacterial 16S rRNA and eukaryotic 18S rRNA genes showed a significant
effect of parasite exposure on bacterial beta-diversity, which explained
between 5.2% and 15.0% of the variation of the bacterial community
structure in the cohort. Additionally, exposure to parasites was
associated with significant changes in the relative abundances of
multiple bacterial taxa, characterized by an increase in Clostridiales
and decreases in Actinobacteria and Bacteroidales. Parasite exposure was
not associated with changes in intestinal eukaryote relative abundances.
However, we found several significant positive correlations between
intestinal bacteria and eukaryotes, including Osciliospira with
Entamoeba coli and Prevotella stercorea with Entamoeba hartmanni, as
well as the co-occurrence of the fungus Candida with Bacteroides and
Actinomyces, Bihdobactedum, and Prevotella copri and the fungus Pichia
with Oscillospira. The parasitic exposure-associated changes in the
bacterial community structure suggest effects on microbial metabolic
routes, host nutrient uptake abilities, and intestinal immunity
regulation in host-parasite interactions.
IMPORTANCE The impact of intestinal eukaryotes on the prokaryotic
microbiome composition of asymptomatic carriers has not been extensively
explored, especially in infants and mothers with multiple parasitic
infections. In this work, we studied the relationship between protist
and helminth parasite colonization and the intestinal microbiota
structure in an asymptomatic population of mother-child binomials from a
semirural community in Mexico. We found that the presence of parasitic
eukaryotes correlated with changes in the bacterial gut community
structure in the intestinal microbiota in an age-dependent way.
Parasitic infection was associated with an increase in the relative
abundance of the class Clostridia and decreases of Actinobacteria and
Bacteroidia. Parasitic infection was not associated with changes in the
eukaryote community structure. However, we observed strong positive
correlations between bacterial and other eukaryote taxa, identifying
novel relationships between prokaryotes and fungi reflecting
interkingdom interactions within the human intestine.
Filiaciones:
Partida-Rodriguez, Oswaldo:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Nieves-Ramirez, Miriam:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Laforest-Lapointe, Isabelle:
Department of Physiology and Pharmacology, University of Calgarygrid.22072.35, Calgary, AB, Canada
Department of Pediatrics, University of Calgarygrid.22072.35, Calgary, AB, Canada
Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada
Brown, Eric M.:
Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Parfrey, Laura:
Department of Zoology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Botany, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Valadez-Salazar, Alicia:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Thorson, Lisa:
Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Moran, Patricia:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Gonzalez, Enrique:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Rascon, Edgar:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Magaña U.:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Hernandez, Eric:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Rojas-Velazquez, Liliana:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Torres, Javier:
Unidad de Investigación en Enfermedades Infecciosas, UMAE Pediatria, IMSSMexico City, Mexico
Arrieta, Marie Claire:
Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Physiology and Pharmacology, University of Calgarygrid.22072.35, Calgary, AB, Canada
Department of Pediatrics, University of Calgarygrid.22072.35, Calgary, AB, Canada
Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Ximenez, Cecilia:
Laboratorio de Inmunología del Unidad de Medicina Experimental, UNAMMexico City, Mexico
Finlay, B. Brett:
Michael Smith Laboratories, Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Biochemistry and Molecular Biology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
Department of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, BC, Canada
gold, Green Published, All Open Access, Gold, Green
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