Endogenous auxin accumulation/localization during zygotic and somatic embryogenesis of Capsicum chinense Jacq
Por:
Pérez-Pastrana J., Testillano P.S., Barany I., Canto-Flick A., Álvarez-López D., Pijeira-Fernández G., Avilés-Viñas S.A., Peña-Yam L., Muñoz-Ramírez L., Nahuat-Dzib S., Islas-Flores I., Santana-Buzzy N.
Publicada:
1 ene 2021
Resumen:
Zygotic and somatic embryogenesis in plants is a fascinating event that is finely regulated through the expression of a specific group of genes and dynamic levels of plant hormones whose concerted action determines the fate that specific cells follow towards zygotic or somatic embryo development. This work studied different stages of Capsicum chinense Jacq. zygotic and somatic embryogenesis. HPLC quantification determined that the levels of indole-3-acetic acid (IAA) increase as the zygotic or somatic embryogenesis progresses, being higher at maturity, thus supporting a positive correlation between embryo cell differentiation and IAA increase. A monoclonal anti-IAA-antibody was used to detect IAA levels. Findings revealed a dynamic pattern of auxin distribution along the different embryogenic embryonic stages. In the early stages of zygotic embryos, the IAA gradient was observed in the basal cells of the suspensor and the hypostases, suggesting that they are the initial source of the IAA hormone. As embryogenesis proceeds, the dynamic of the IAA gradient is displaced to the embryo and endosperm cells. In the case of induced somatic embryogenesis, the IAA gradient was detected in the dividing cells of the endodermis, from where pre-embryogenic cells emerge. However, the analysis of somatic embryos revealed that IAA was homogeneously distributed. This study shows evidence supporting a correlation between IAA levels during zygotic or somatic embryogenesis in Capsicum chinense species. © 2021 Elsevier GmbH
Filiaciones:
Pérez-Pastrana J.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Testillano P.S.:
Pollen biotechnology of crop plants group, Centro de Investigaciones Biológicas-CSIC, Ramiro Maeztu 9, Madrid, 28040, Spain
Barany I.:
Pollen biotechnology of crop plants group, Centro de Investigaciones Biológicas-CSIC, Ramiro Maeztu 9, Madrid, 28040, Spain
Canto-Flick A.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Álvarez-López D.:
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 x 32 y 34, colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97200, Mexico
Pijeira-Fernández G.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Avilés-Viñas S.A.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Peña-Yam L.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Muñoz-Ramírez L.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Nahuat-Dzib S.:
Laboratorio de Biotecnología, Departamento Ingeniería Química-Bioquímica, TecNM/ Instituto Tecnológico de Mérida, Mexico
Islas-Flores I.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
Santana-Buzzy N.:
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida, Yucatán C.P. 97205, Mexico
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