Assessment of Cytogenetic Damage and Cholinesterases' Activity in Workers Occupationally Exposed to Pesticides in Zamora-Jacona, Michoacan, Mexico
Por:
Valencia-Quintana, Rafael, Lopez-Duran, Rosa Maria, Milic, Mirta, Bonassi, Stefano, Ochoa-Ocana, Ma. Antonieta, Uriostegui-Acosta, Mayrut Osdely, Perez-Flores, Guillermo Alejandro, Gomez-Olivares, Jose Luis, Sanchez-Alarcon, Juana
Publicada:
1 jun 2021
Resumen:
Pesticides have been considered as potential chemical mutagens; however,
little is known about toxic and genotoxic effects during pesticide
application in Zamora-Jacona, Michoacan State in Mexico. This study
sought to determine DNA damage and cholinesterase activities inhibitions
in 54 agricultural workers exposed to complex mixtures of pesticides vs.
control group (26 individuals) using Comet assay in peripheral whole
blood, micronucleus (MN) test in oral mucosa cells, Cytokinesis-blocked
MN assay in lymphocytes (L-CBMNcyt) and measuring AChE and BChE
activities in whole blood and plasma samples, respectively. Exposed
subjects demonstrated significantly elevated levels of primary (Comet
assay: tail intensity, tail length, tail moment, Olive tail moment) and
permanent DNA damage (MN assay: in blood/buccal cells; frequencies of
nuclear buds, binucleated cells, cells with condensed chromatin,
karyorrhexis, pyknosis, and karyolysis). However, inhibition of
cholinesterase activities (AChE and BChE) was not observed in the
workers. Confounding factors including sex, age, BMI, working exposure
period, protection level, smoking habit (cigarettes per day units),
alcohol consumption (weekly), medication, were considered in the
analysis. These combined techniques demonstrated usefulness in the
health hazards risks pesticide exposure assessment and suggested the
need for periodic monitoring together with the education and the
training of occupational workers for the safe application of potentially
harmful pesticides.
Filiaciones:
Sanchez-Alarcon, J (Corresponding Author), Univ Autonoma Tlaxcala, Lab Rafael Villalobos Pietrini Toxicol Genom & Qu, Fac Agrobiol, CA Genet & Ambiente UATLX CA 223,Red Temat Toxico, Tlaxcala 90120, Mexico. Lopez-Duran, RM (Corresponding Author), Univ Autonoma Metropolitana Iztapalapa, Div Ciencias Biol & Salud, Lab Biomembranas, Ciudad De Mexico 09340, Mexico. Valencia-Quintana, Rafael
Open Access
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