Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination
Por:
Farias-Serratos, Brenda Minerva, Lazcano, Ivan, Villalobos, Patricia, Darras, Veerle M., Orozco, Aurea
Publicada:
1 ene 2021
Categoría:
Multidisciplinary
Resumen:
Thyroid hormones are messengers that bind to specific nuclear receptors
and regulate a wide range of physiological processes in the early stages
of vertebrate embryonic development, including neurodevelopment and
myelogenesis. We here tested the effects of reduced T3 availability upon
the myelination process by treating zebrafish embryos with low
concentrations of iopanoic acid (IOP) to block T4 to T3 conversion.
Black Gold II staining showed that T3 deficiency reduced the myelin
density in the forebrain, midbrain, hindbrain and the spinal cord at 3
and 7 dpf. These observations were confirmed in 3 dpf mbp:egfp
transgenic zebrafish, showing that the administration of IOP reduced the
fluorescent signal in the brain. T3 rescue treatment restored brain
myelination and reversed the changes in myelin-related gene expression
induced by IOP exposure. NG2 immunostaining revealed that T3 deficiency
reduced the amount of oligodendrocyte precursor cells in 3 dpf
IOP-treated larvae. Altogether, the present results show that inhibition
of T4 to T3 conversion results in hypomyelination, suggesting that THs
are part of the key signaling molecules that control the timing of
oligodendrocyte differentiation and myelin synthesis from very early
stages of brain development.
Filiaciones:
Farias-Serratos, Brenda Minerva:
Instituto de Neurobiologia, Universidad Nacional Autónoma de México (UNAM), Qro, Queretaro, Mexico
Lazcano, Ivan:
Instituto de Neurobiologia, Universidad Nacional Autónoma de México (UNAM), Qro, Queretaro, Mexico
Villalobos, Patricia:
Instituto de Neurobiologia, Universidad Nacional Autónoma de México (UNAM), Qro, Queretaro, Mexico
Darras, Veerle M.:
Instituto de Neurobiologia, Universidad Nacional Autónoma de México (UNAM), Qro, Queretaro, Mexico
Biology Department, Laboratory of Comparative Endocrinology, KU Leuven, Leuven, Belgium
Orozco, Aurea:
Instituto de Neurobiologia, Universidad Nacional Autónoma de México (UNAM), Qro, Queretaro, Mexico
All Open Access; Gold
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