Temporal Dynamics of T Helper Populations in the Proximal Small Intestine after Oral Bovine Lactoferrin Administration in BALB/c Mice
Por:
Ynga-Durand, Mario, Tapia-Pastrana, Gabriela, Rebollar-Ruiz, Xochitl Abril, Yepez-Ortega, Mariazell, Nieto-Yanez, Oscar, Arciniega-Martinez, Ivonne Maciel, Resendiz-Albor, Aldo Arturo
Publicada:
1 ago 2021
Resumen:
Bovine lactoferrin (bLf), a component of milk and a dietary supplement,
modulates intestinal immunity at effector and inductor sites.
Considering the regional difference in intestinal compartments and the
dynamics of local cytokine-producing cells in the gut across time, the
aim of this work was to characterize the effects of bLf on the proximal
small intestine in a BALB/c murine model of oral administration. Male
BALB/c mice were treated with oral bLf vs. saline control as mock by
buccal deposition for 28 days. Intestinal secretions were obtained at
different time points and cells were isolated from Peyer's patches (PP)
and lamina propria (LP) of the proximal small intestine as
representative inductor and effector sites, respectively. Total and
specific anti-bLF IgA and IgM were determined by enzyme-immuno assay;
the percentages of IgA(+) and IgM(+) plasma cells (PC) and
cytokine-producing CD4(+) T cells of PP and LP were analyzed by flow
cytometry. We found that total and bLf-specific IgA and IgM levels were
increased in the intestinal secretions of the bLf group in comparison to
mock group and day 0. LP IgA(+) PC and IgM(+) PC presented an initial
elevation on day 7 and day 21, respectively, followed by a decrease on
day 28 in comparison to mock. Higher percentages of CD4(+) T cells in LP
were found in the bLf group. Cytokines-producing CD4(+) T cells
populations presented a pattern of increases and decreases in the bLf
group in both LP and PP. Transforming growth factor beta (TGF-beta)(+)
CD4(+) T cells showed higher percentages after bLf administration with a
marked peak at day 21 in both LP and PP in comparison to mock-treated
mice. Oral bLf exhibits complex immune properties in the proximal small
intestine, where temporal monitoring of the inductor and effector
compartments reveals patterns of rises and falls of different cell
populations. Exceptionally, TGF-beta(+) CD4(+) T cells show consistent
higher numbers after bLf intervention across time. Our work suggests
that isolated measurements do not show the complete picture of the
modulatory effects of oral bLf in immunological sites as dynamic as the
proximal small intestine.
Filiaciones:
Ynga-Durand, Mario:
Laboratorio de Inmunidad de Mucosas, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Salvador Díaz Mirón y Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, México City, 11340, Mexico
Tapia-Pastrana, Gabriela:
Laboratorio de Investigación Biomédica del Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, 71256, Mexico
Rebollar-Ruiz, Xochitl Abril:
Laboratorio de Inmunidad de Mucosas, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Salvador Díaz Mirón y Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, México City, 11340, Mexico
Yepez-Ortega, Mariazell:
Laboratorio de Inmunidad de Mucosas, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Salvador Díaz Mirón y Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, México City, 11340, Mexico
Nieto-Yanez, Oscar:
Carrera de Médico Cirujano, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, 54090, Mexico
Arciniega-Martinez, Ivonne Maciel:
Laboratorio de Inmunidad de Mucosas, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Salvador Díaz Mirón y Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, México City, 11340, Mexico
Resendiz-Albor, Aldo Arturo:
Laboratorio de Inmunidad de Mucosas, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Salvador Díaz Mirón y Plan de San Luis S/N, Miguel Hidalgo, Casco de Santo Tomas, México City, 11340, Mexico
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