Bacteria cultured from the gut of Meccus pallidipennis (Hemiptera: Reduviidae), a triatomine species endemic to Mexico
Por:
Jimenez-Cortes, J. G., Garcia-Contreras, R., Bucio-Torres, I, M., Cabrera-Bravo, M., Lopez-Jacome, L. E., Franco-Cendejas, R., Vences-Blanco, M. O., Salazar-Schettino, P. M.
Publicada:
1 sep 2021
Ahead of Print:
1 dic 2020
Resumen:
The study of intestinal microbiota in vector insects like triatomines is
paramount in parasitology because many parasitic species inhabit the
vector's gut. Although knowledge on the gut microbiota in various
vectors of the parasitic flagellate Trypanosoma cruzi has grown,
research efforts have focused on South American triatomines. This study
reports the isolation of bacterial microbiota in the anterior and
posterior gut of Meccus pallidipennis (a triatomine species endemic to
Mexico) by culture, as well as its identification by phenotypic and
biochemical tests and its quantification by counting colony-forming
units. The study was performed on fifth-instar nymph and adult specimens
of M. pallidipennis, either laboratory-bred or collected in the field
and either infected or not with T. cruzi. Overall, 17 bacterial species
were identified, with the genera Bacillus and Staphylococcus being the
most prevalent regardless of the origin of the insects. No differences
were observed in the number of bacterial species in the gut of
laboratory-bred and field-collected insects, neither with respect to
life stage or infection status. In general, the Shannon-Weaver diversity
index was higher in non-infected insects than in infected ones. Further
studies using non-culture methods are required to determine whether
bacterial species diversity is modified by laboratory breeding.
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