Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
Por:
Leon-Mancilla, Benjamin, Martinez-Castillo, Moises, Medina-Avila, Zaira, Perez-Torres, Armando, Garcia-Loya, Jorge, Alfaro-Cruz, Ana, Pina-Barba, Cristina, Gutierrez-Reyes, Gabriela
Publicada:
1 jun 2021
Resumen:
Liver diseases are the leading cause of death worldwide. Excessive
alcohol consumption, a high-fat diet, and hepatitis C virus infection
promote fibrosis, cirrhosis, and/or hepatocellular carcinoma. Liver
transplantation is the clinically recommended procedure to improve and
extend the life span of patients in advanced disease stages. However,
only 10% of transplants are successful, with organ availability,
presurgical and postsurgical procedures, and elevated costs directly
correlated with that result. Extracellular matrix (ECM) scaffolds have
emerged as an alternative for tissue restoration. Biocompatibility and
graft acceptance are the main beneficial characteristics of those
biomaterials. Although the capacity to restore the size and correct
function of the liver has been evaluated in liver hepatectomy models,
the use of scaffolds or some kind of support to replace the volume of
the extirpated liver mass has not been assessed.
Partial hepatectomy was performed in a rat liver with the
xenoimplantation of a collagen matrix scaffold (CMS) from a bovine
condyle. Left liver lobe tissue was removed (approximately 40%), and an
equal proportion of CMS was surgically implanted. Liver function tests
were evaluated before and after the surgical procedure. After days 3,
14, and 21, the animals were euthanized, and macroscopic and histologic
evaluations were performed. On days 3 and 14, adipose tissue was
observed surrounding the CMS, with no clinical evidence of rejection or
infection, as was vessel neoformation and CMS reabsorption at day 21.
There was histologic evidence of an insignificant inflammation process
and migration of adjacent cells to the CMS, observed with the
hematoxylin and eosin (H&E) and Masson's trichrome staining. The CMS
was shown to perform well in liver tissue and could be a useful
alternative for studying tissue regeneration and repair in chronic liver
diseases.
Filiaciones:
Leon-Mancilla, Benjamin:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Martinez-Castillo, Moises:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Medina-Avila, Zaira:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Perez-Torres, Armando:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Garcia-Loya, Jorge:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Alfaro-Cruz, Ana:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Pina-Barba, Cristina:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
Gutierrez-Reyes, Gabriela:
Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Liver Pancreas & Motil Lab, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Unit Expt Med, Mexico City, DF, Mexico. Gutierrez-Reyes, G (Corresponding Author), Univ Nacl Autonoma Mexico, Sch Med, Mexico City, DF, Mexico. Leon-Mancilla, Benjamin
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