In Vivo Study on Bone Response to 3D-Printed Constructs Designed from Microtomographic Images
Por:
Perez-Sanchez, Lucia, De la O, Misael Aaron Ortiz, Gonzalez-Alva, Patricia, MEDINA, LUIS ALBERTO, Masuoka-Ito, David, alvarez-Perez, Marco Antonio, Serrano-Bello, Janeth
Publicada:
1 jul 2021
Ahead of Print:
1 mar 2021
Resumen:
The design of scaffolds that could adjust and adapt greatly to bone
defects is a significant challenge to bone tissue engineering. Recently,
3D printing technology emerged as a process that could precisely control
the architecture and design an exact geometry of the scaffold to the
site or defect where it will be implanted. Thus, this research aimed to
design and synthesize individualized constructs of polylactic acid (PLA)
by 3D printing using microtomographic images to fit the edge of Wistar
rat calvaria's critical size defects. The 3D-printed construct using the
DICOM data of microtomographic images process to STL manipulated and
designed by various software showed an excellent geometry. The in vitro
biocompatibility assay of the 3D printing scaffold was evaluated by
WST-1, showing an excellent biological response. Moreover, the in vivo
evaluation of the bone regeneration process onto the rat calvaria defect
model measured by the bone mineral density (BMD) at 8, 30, 60, and 90
days via micro-CT showed that at the end of the evaluation period, the
3D construct was integrated into the edges of the bone tissue, and new
tissue deposited was in the process of mineralization. These findings
suggest that the 3D construct matches the calvaria defect, allowing the
novo mineral tissue to form. These individualized printed scaffolds may
be a promising candidate in bone tissue engineering for future
regeneration strategies.
Filiaciones:
Perez-Sanchez, Lucia:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
De la O, Misael Aaron Ortiz:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
Gonzalez-Alva, Patricia:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
MEDINA, LUIS ALBERTO:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
Masuoka-Ito, David:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
alvarez-Perez, Marco Antonio:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
Serrano-Bello, Janeth:
Alvarez-Perez, MA (Corresponding Author), Natl Autonomous Univ Mexico UNAM, Fac Dent, Postgrad Studies & Res Div, Tissue Bioengn Lab, Mexico City 04510, DF, Mexico. Perez-Sanchez, Lucia
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