Photoautotrophic poly(3-hydroxybutyrate) production by a wild-type Synechococcus elongatus isolated from an extreme environment
Por:
Gonzalez-Resendiz, Laura, Sanchez-Garcia, Leon, Hernandez-Martinez, Ingrid, Vigueras-Ramirez, Gabriel, Felipe Jimenez-Garcia, Luis, Lara-Martinez, Reyna, Morales-Ibarria, Marcia
Publicada:
1 oct 2021
Resumen:
The photoautotrophic poly(3-hydroxybutyrate) (PHB) production by
cyanobacteria is an attractive option as it only requires CO2 and light.
In this work, a new wild-type strain producing PHB, Synechococcus
elongatus UAM-C/S03, was identified using a polyphasic approach. The
strain was cultured in a photobioreactor operated under N-sufficiency
conditions at different pH values (7 to 11) and fed with CO2 on demand.
We also evaluated the production of PHB under N-starving conditions.
Highest biomass productivity, 324 mg L-1 d-1, and CO2 capture, 674 mg
L-1 d-1, were obtained at pH 7 and under N-sufficiency conditions. The
strain accumulated 29.42% of PHB in dry cell weight (DCW) under
N-starvation conditions without pH control, and highest PHB productivity
was 58.10 mg L-1 d-1. The highest carbohydrate content registered at pH
8, 50.84% in DCW, along with a release of carbon-based organic
compounds, suggested the presence of exopolysaccharides in the culture
medium.
Filiaciones:
Gonzalez-Resendiz, Laura:
Univ Autonoma Metropolitana Cuajimalpa, Dept Proc & Tecnol, Av Vasco de Quiroga 4871, Cd De Mexico 05348, Mexico
Sanchez-Garcia, Leon:
Univ Autonoma Metropolitana Iztapalapa, Biotecnol, San Rafael Atlixco 186, Cd De Mexico 09340, Mexico
Hernandez-Martinez, Ingrid:
Univ Autonoma Metropolitana Cuajimalpa, Ciencias Nat & Ingn, Av Vasco de Quiroga 4871, Cd De Mexico Cp 05348, Mexico
Vigueras-Ramirez, Gabriel:
Univ Autonoma Metropolitana Cuajimalpa, Dept Proc & Tecnol, Av Vasco de Quiroga 4871, Cd De Mexico 05348, Mexico
Felipe Jimenez-Garcia, Luis:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Circuito Exterior Ciudad Univ, Cd De Mexico 04510, Mexico
Lara-Martinez, Reyna:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Circuito Exterior Ciudad Univ, Cd De Mexico 04510, Mexico
Morales-Ibarria, Marcia:
Univ Autonoma Metropolitana Cuajimalpa, Dept Proc & Tecnol, Av Vasco de Quiroga 4871, Cd De Mexico 05348, Mexico
|