Effects of Octenidine on the Formation and Disruption of Dental Biofilms: An Exploratory In Situ Study in Healthy Subjects
Por:
Reda, B., Dudek, J., Martinez-Hernandez, M., Hannig, M.
Publicada:
1 ago 2021
Categoría:
Dentistry (miscellaneous)
Resumen:
Dental biofilms are highly structured, complex multispecies communities
that, if left untreated, lead to severe oral complications such as
caries and periodontal diseases. Therefore, antibiofilm agents are often
recommended for both preventive and therapeutic measures. However,
biofilm management can be challenging due to the low sensitivity of
biofilms to antimicrobial treatments. Octenidine dihydrochloride (OCT)
is a highly effective antibacterial agent. Because the OCT antibiofilm
efficacy has not been studied in situ, this exploratory crossover study
aimed to evaluate the effects of OCT mouth rinsing on biofilm formation
and on the disruption of mature biofilms. Moreover, a comparison to the
gold-standard chlorhexidine (CHX) was conducted. The biofilms were
formed intraorally by 5 healthy volunteers on enamel specimens fixed to
acrylic splints. For biofilm formation analysis, OCT, CHX, or water
rinses were applied for 30 s every 12 h. The samples evaluation took
place at 24-and 48-h time points. For biofilm disruption analysis,
sample assessment was performed before and directly after the first OCT
or CHX rinse on 48-h mature biofilms. A second rinse was carried out 12
h later. The last assessment was applied to 72-h mature biofilms. The
biofilms were analyzed by fluorescence microscopy and transmission
electron microscopy. The results showed OCT significantly reducing
biofilm formation and bacterial vitality in situ. Simultaneously, the
biofilm thickness was strongly decreased. Moreover, a single application
of OCT to a 48-h mature biofilm induced substantial biofilm disruption.
In addition, the efficacy of OCT compared favorably to CHX. These
findings show that OCT rinses prevent biofilm formation and disrupt
preexisting mature biofilms formed by healthy subjects. This work
suggests that OCT might be used for dental biofilm management as a part
of the medical treatment of oral diseases. Future studies with a larger
subject heterogeneity and number are needed to confirm the observed OCT
effects.
Filiaciones:
Saarland Univ, Clin Operat Dent Periodontol & Prevent Dent, Kirrberger Str 100, D-66421 Homburg, Saarland, Germany
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Circuito Exterior S-N,Ciudad Univ, Mexico City, DF, Mexico
(Corresponding Author), Saarland Univ, Clin Operat Dent Periodontol & Prevent Dent, Kirrberger Str 100, D-66421 Homburg, Saarland, Germany
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