Study of the ultrastructure of Enterococcus faecalis and Streptococcus mutans incubated with salivary antimicrobial peptides
Por:
Blancas, Blanca, Lanzagorta, Maria de Lourdes, Jimenez-Garcia, Luis Felipe, Lara, Reyna, MOLINARI, JOSE LUIS, FERNANDEZ, ANA MARIA
Publicada:
1 jun 2021
Ahead of Print:
1 may 2021
Categoría:
Dentistry (miscellaneous)
Resumen:
Objectives: Enterococcus faecalis has been associated with root canal
infections, while Streptococcus mutans has a central role in the
etiology of dental caries. One of the main reasons of endodontic failure
has been associated to the presence of E. faecalis and the formation of
biofilms. S. mutans inhabits the oral cavity, specifically the dental
plaque, which is a multispecies biofilm formed on the hard surfaces of
the tooth. The biofilm formation is the main factor determining the
pathogenicity of numerous bacteria. Natural antimicrobial peptides in
the saliva protect against pathogenic bacteria and biofilms. The aim of
this study was to assess the ultrastructural damage induced by salivary
peptides in bacteria involved in biofilms has not been previously
studied.
Material and methods: Enterococcus faecalis and S. mutans incubated with
cystatin C, chromogranin A, or histatin 5 were morphologically analyzed
and counted. The ultrastructural damage was evaluated by transmission
electron microscopy (TEM).
Results: A decrease in bacterial numbers was observed after incubation
with cystatin C, chromogranin A, or histatin 5, compared to the control
group (P < 0.001). Ultrastructural damage in E. faecalis and S. mutans
incubated with salivary peptides was found in the cell wall, plasma
membrane with a decreased distance between the bilayers, a granular
pattern in the cytoplasm, and pyknotic nucleoids.
Conclusions: This study demonstrated that salivary peptides exert
antibacterial activity and induce morphological damage on E. faecalis
and S. mutans. Knowledge on the ultrastructural damage inflicted by
salivary antimicrobial peptides on two important bacteria causing dental
caries and root canal infections could aid the design of new therapeutic
approaches to facilitate the elimination of these bacteria.
Filiaciones:
Blancas, Blanca:
Col Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Av Univ 3000, Mexico City 04510, DF, Mexico
Lanzagorta, Maria de Lourdes:
Inst Estudios Avanzados Odontol Dr Yury Kuttler, Maestria Endodoncia, Mexico City, DF, Mexico
Jimenez-Garcia, Luis Felipe:
Univ Nacl Autonoma Mexico, Col Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City, DF, Mexico
Lara, Reyna:
Univ Nacl Autonoma Mexico, Col Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City, DF, Mexico
MOLINARI, JOSE LUIS:
Col Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Bioquim & Biol Estruct, Mexico City, DF, Mexico
FERNANDEZ, ANA MARIA:
Col Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Av Univ 3000, Mexico City 04510, DF, Mexico
Inst Estudios Avanzados Odontol Dr Yury Kuttler, Maestria Endodoncia, Mexico City, DF, Mexico
Univ Anahuac Mexico Campus Norte, Ctr Invest Ciencias Salud CICSA, FCS, Huixquilucan, Mexico
Gold, Green Published, Green
|