Exploring the genetic structure of Parastrigea diovadena Dubois and Macko, 1972 (Digenea: Strigeidae), an endoparasite of the white ibis, Eudocimus albus, from the Neotropical region of Mexico
Por:
Lopez-Jimenez, Alejandra, Hernandez-Mena, David Ivan, Solorzano-Garcia, Brenda, Garcia-Varela, Martin
Publicada:
1 jun 2021
Ahead of Print:
1 may 2021
Resumen:
Parastrigea diovadena Dubois and Macko, 1972, is an allogenic trematode
species that infects the intestine of white ibis. This widely
distributed Neotropical species has been studied poorly, and nothing is
known about its population genetic structure. In the current study, we
attempt to fill this gap for the first time and to explore the genetic
diversity in P. diovadena populations from three biogeographic provinces
(Sierra Madre Oriental, Sierra Madre Occidental, and Sierra Madre del
Sur) in the Neotropical region of Mexico. Newly generated sequences of
the internal transcribed spacers (ITS) from ribosomal DNA and cytochrome
c oxidase subunit 1 (cox 1) from mitochondrial DNA were compared with
sequences available from the GenBank data set. Phylogenetic analyses
performed with the ITS and cox 1 data sets using maximum likelihood and
Bayesian inference unequivocally showed that new sequences of P.
diovadena recovered from the white ibis formed a clade with other
sequences of specimens previously identified as P. diovadena. The
intraspecific genetic divergence among the isolates was very low,
ranging from 0 to 0.38% for ITS and from 0 to 1.5% for cox 1, and in
combination with the phylogenetic trees confirmed that the isolates
belonged to the same species. The cox 1 haplotype network (star-shaped)
inferred with 62 sequences revealed 36 haplotypes. The most frequent
haplotype (H3, n = 18) corresponded to specimens from all the
populations (except Tecolutla, Veracruz). In addition to the common
haplotype, we identified four other shared haplotypes (H2, H9, H12, and
H14) and 31 unique haplotypes (singlets). In addition, high haplotype
diversity (Hd = 0.913), low nucleotide diversity (Pi = 0.0057), and null
genetic differentiation or population structure (Fst = 0.0167) were
found among the populations from the three biogeographic provinces. The
results suggest that the biology of the definitive host has played a key
role in the population genetic structure of Parastrigea diovadena in the
Neotropical region of Mexico.
Filiaciones:
Lopez-Jimenez, Alejandra:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencias Biol, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Hernandez-Mena, David Ivan:
Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Antigua Carretera Progreso Km 6, Merida 97310, Yucatan, Mexico
Solorzano-Garcia, Brenda:
Univ Nacl Autonoma Mexico ENES Merida, Unidad Merida, Escuela Nacl Estudios Super, Km 4-5 Carretera Merida Tetiz, Ucu, Yucatan, Mexico
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
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