Genetic diversity ofBorrelia burgdorferisensu stricto: Novel strains from Mexican wild rodents
Por:
Colunga-Salas, Pablo, Hernandez-Canchola, Giovani, Sanchez-Montes, Sokani, Lozano-Sardaneta, Yokomi N., Becker, Ingeborg
Publicada:
1 may 2021
Ahead of Print:
1 sep 2020
Resumen:
Borrelia burgdorferis.s. is a Gram-negative spirochaete, the
aetiological agent of Lyme disease, the most common vector-borne disease
in the Northern hemisphere. Reports on the presence ofB. burgdorferiin
central Mexico have been strongly criticized, since these were based
only on unspecific serological methods. Furthermore, the worldwide
genetic diversity ofB. burgdorferis.s. has not been evaluated. For this
reason, the aim of the present study was to confirm the presence ofB.
burgdorferiin the central area of Mexico and to evaluate its
relationship with regard to the global genetic diversity ofB.
burgdorferis.s. To achieve this, fragments of theflagellinand theouter
surface proteinA genes were amplified from ear biopsies of the arboreal
wild endemic miceHabromys schmidlyi. With these sequences, a
concatenated Bayesian analysis was performed to confirm the identity
ofB. burgdorferis.s. Afterwards, the global genetic diversity of this
bacterial species was evaluated using our sequences and those available
in GenBank. A prevalence of 10.4% (5/48) ofH. schmidlyiinfected
withBorreliasp. was detected, and the phylogenetic analyses confirmed
the identity ofB. burgdorferis.s. Using both genes, the genetic
diversity was low. However, genetic structuring analyses revealed that
populations of western United States and those from Mexico formed
slightly different genetic groups, separated from the populations of the
rest of the world. Our study not only confirms the presence of this
bacterium in central Mexico, but also shows the most southern record of
this bacterium so far. It also highlights the importance ofH.
schmidlyias a new potential host of this bacterial species. Our study
also provides first genetic data on an incipient process of divergence
inB. burgdorferis.s. populations of eastern United States and central
Mexico.
Filiaciones:
Colunga-Salas, Pablo:
Univ Nacl Autonoma Mexico, Fac Med, Ctr Med Trop, Div Invest, Ciudad De Mexico, Mexico
Univ Nacl Autonoma Mexico, Programa Doctorado Ciencias Biomed, Ciudad De Mexico, Mexico
Hernandez-Canchola, Giovani:
Louisiana State Univ, Museum Nat Sci, 119 Foster Hall, Baton Rouge, LA 70803 USA
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Evolut, Colecc Mamiferos,Museo Zool Alfonso L Herrera, Ciudad De Mexico, Mexico
Sanchez-Montes, Sokani:
Univ Veracruzana, Fac Ciencias Biol & Agr Reg Tuxpan, Xalapa, Veracruz, Mexico
Lozano-Sardaneta, Yokomi N.:
Univ Nacl Autonoma Mexico, Fac Med, Ctr Med Trop, Div Invest, Ciudad De Mexico, Mexico
Becker, Ingeborg:
Univ Nacl Autonoma Mexico, Fac Med, Ctr Med Trop, Div Invest, Ciudad De Mexico, Mexico
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