Sleep-wake cycle disturbances and NeuN-altered expression in adult rats after cannabidiol treatments during adolescence
Por:
Murillo-Rodriguez, Eric, Millan-Aldaco, Diana, Cicconcelli, Dorotea, Giorgetti, Valentina, Arankowsky-Sandoval, Gloria, Alcaraz-Silva, Jocelyne, Imperatori, Claudio, Machado, Sergio, Budde, Henning, Torterolo, Pablo
Publicada:
1 jun 2021
Ahead of Print:
1 feb 2021
Categoría:
Pharmacology
Resumen:
Rationale The medical uses of cannabidiol (CBD), a constituent of the
Cannabis sativa, have accelerated the legal and social acceptance for
CBD-based medications but has also given the momentum for questioning
whether the long-term use of CBD during the early years of life may
induce adverse neurobiological effects in adulthood, including sleep
disturbances. Given the critical window for neuroplasticity and
neuro-functional changes that occur during stages of adolescence, we
hypothesized that CBD might influence the sleep-wake cycle in adult rats
after their exposure to CBD during the adolescence. Objectives Here, we
investigated the effects upon behavior and neural activity in adulthood
after long-term administrations of CBD in juvenile rats. Methods We
pre-treated juvenile rats with CBD (5 or 30 mg/Kg, daily) from
post-natal day (PND) 30 and during 2 weeks. Following the treatments,
the sleep-wake cycle and NeuN expression was analyzed at PND 80. Results
We found that systemic injections of CBD (5 or 30 mg/Kg, i.p.) given to
adolescent rats (post-natal day 30) for 14 days increased in adulthood
the wakefulness and decreased rapid eye movement sleep during the
lights-on period whereas across the lights-off period, wakefulness was
diminished and slow wave sleep was enhanced. In addition, we found that
adult animals that received CBD during the adolescence displayed
disruptions in sleep rebound period after total sleep deprivation.
Finally, we determined how the chronic administrations of CBD during the
adolescence affected in the adulthood the NeuN expression in the
suprachiasmatic nucleus, a sleep-related brain region. Conclusions Our
findings are relevant for interpreting results of adult rats that were
chronically exposed to CBD during the adolescence and provide new
insights into how CBD may impact the sleep-wake cycle and neuronal
activity during developmental stages.
Filiaciones:
Murillo-Rodriguez, Eric:
Univ Anahuac Mayab Merida, Div Ciencias Salud, Lab Neurociencias Mol & Integrat, Escuela Med, Km 15-5 Carretera Merida Progreso, Merida 97308, Yucatan, Mexico
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Millan-Aldaco, Diana:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Div Neurociencias, Ciudad De Mexico, Mexico
Cicconcelli, Dorotea:
European Univ Rome, Dept Human Sci, Cognit & Clin Psychol Lab, Rome, Italy
Giorgetti, Valentina:
European Univ Rome, Dept Human Sci, Cognit & Clin Psychol Lab, Rome, Italy
Arankowsky-Sandoval, Gloria:
Univ Autonoma Yucatan, Ctr Invest Reg Dr Hideyo Noguchi, Merida, Yucatan, Mexico
Alcaraz-Silva, Jocelyne:
Univ Anahuac Mayab Merida, Div Ciencias Salud, Lab Neurociencias Mol & Integrat, Escuela Med, Km 15-5 Carretera Merida Progreso, Merida 97308, Yucatan, Mexico
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Imperatori, Claudio:
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
European Univ Rome, Dept Human Sci, Cognit & Clin Psychol Lab, Rome, Italy
Machado, Sergio:
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Univ Salgado de Oliveira, Lab Phys Act Neurosci, Phys Act Sci Postgrad Program, Niteroi, RJ, Brazil
Neurodivers Inst, Lab Phys Act Neurosci, Queimados, RJ, Brazil
Budde, Henning:
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Med Sch Hamburg, Fac Human Sci, Hamburg, Germany
Torterolo, Pablo:
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Univ Republica, Fac Med, Lab Neurobiol Sueno, Dept Fisiol, Montevideo, Uruguay
|