SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
Por:
Lizarraga-Verdugo, Erik, Carmona, Tatiana Galicia, Ramos-Payan, Rosalio, Avendano-Felix, Mariana, Bermudez, Mercedes, Parra-Niebla, Maryelv, Lopez-Camarillo, Cesar, Fernandez-Figueroa, Edith, Lino-Silva, Leonardo, Saavedra, Herminia Arvelo, Vela-Sarmiento, Itzel, Ovando, Roberto Cardenas, Ruiz-Garcia, Erika, Aguilar-Medina, Maribel
Publicada:
1 jul 2021
Resumen:
Colorectal cancer (CRC) is one of the most common malignancies worldwide
and includes colon cancer (CC) and rectal cancer (RC). Regarding CC, the
development of novel molecular biomarkers for the accurate diagnosis and
prognosis, as well as the identification of novel targets for
therapeutic intervention, are urgently needed. SRY-related high-mobility
group box 9 (SOX9), a transcription factor, is involved in development,
and has been associated with the progression of human cancer. However,
its underlying clinical and functional effects in CRC have not been
fully understood. Therefore, the present study aimed to evaluate the
clinical and functional relevance of SOX9 expression in CC. The
expression of SOX9 in tumor tissues was evaluated in 97 biopsies from
Mexican patients with CC with early-stage I and II disease by
immunohistochemistry (IHC). In addition, SOX9 silencing in the HCT116
cell line was performed using specific small interfering RNAs, while
downregulation efficiency was verified by reverse
transcription-quantitative PCR and immunofluorescence.
Spheroid-formation assay was carried out using ultra-low attachment
plates. The IHC results showed that SOX9 was upregulated in patients
with stage II (91%) and advanced T3 stage (67%) CC. Interestingly,
higher SOX9 expression was associated with clinical stage, tumor size
and tumor location. Furthermore, increased SOX9 expression was found in
relapsed cases with local tumors; however, it was not associated with
increased survival probability. Additionally, functional analysis
indicated that SOX9 silencing significantly attenuated the
sphere-formation capability of HCT116 cells. The present study was the
first to evaluate the expression levels of SOX9 in Mexican patients
diagnosed with early-stage CC. The aforementioned findings indicated
that high SOX9 expression could play an important role in tumorigenesis
and be associated with advanced T-stages of clinical-stage II patients,
but not with relapse-free survival.
Filiaciones:
Lizarraga-Verdugo, Erik:
Autonomous Univ Sinaloa, Fac Chem & Biol Sci, Josefa Ortiz de Dominguez S-N & Ave Americas, Culiacan 80010, Sinaloa, Mexico
Carmona, Tatiana Galicia:
Natl Canc Inst, Dept Translat Med, Ave San Fernando 22,Belisario Dominguez Secc 16, Mexico City 14080, DF, Mexico
Ramos-Payan, Rosalio:
Autonomous Univ Sinaloa, Fac Chem & Biol Sci, Josefa Ortiz de Dominguez S-N & Ave Americas, Culiacan 80010, Sinaloa, Mexico
Avendano-Felix, Mariana:
Autonomous Univ Sinaloa, Fac Chem & Biol Sci, Josefa Ortiz de Dominguez S-N & Ave Americas, Culiacan 80010, Sinaloa, Mexico
Bermudez, Mercedes:
Autonomous Univ Sinaloa, Fac Chem & Biol Sci, Josefa Ortiz de Dominguez S-N & Ave Americas, Culiacan 80010, Sinaloa, Mexico
Parra-Niebla, Maryelv:
Autonomous Univ Sinaloa, Fac Chem & Biol Sci, Josefa Ortiz de Dominguez S-N & Ave Americas, Culiacan 80010, Sinaloa, Mexico
Lopez-Camarillo, Cesar:
Autonomous Univ Mexico City, Oncogen Lab, Mexico City 06720, DF, Mexico
Fernandez-Figueroa, Edith:
Natl Canc Inst, Dept Computat Genom Labs, Mexico City 14080, DF, Mexico
Lino-Silva, Leonardo:
Natl Canc Inst, Dept Pathol, Mexico City 14080, DF, Mexico
Saavedra, Herminia Arvelo:
Natl Canc Inst, Dept Translat Med, Ave San Fernando 22,Belisario Dominguez Secc 16, Mexico City 14080, DF, Mexico
Vela-Sarmiento, Itzel:
Natl Canc Inst, Dept Gastrointestinal Tumors, Mexico City 14080, DF, Mexico
Ovando, Roberto Cardenas:
Monterrreys Technol, Sch Engn & Sci, Mexico City 14380, DF, Mexico
Ruiz-Garcia, Erika:
Natl Canc Inst, Dept Translat Med, Ave San Fernando 22,Belisario Dominguez Secc 16, Mexico City 14080, DF, Mexico
Aguilar-Medina, Maribel:
Autonomous Univ Sinaloa, Fac Chem & Biol Sci, Josefa Ortiz de Dominguez S-N & Ave Americas, Culiacan 80010, Sinaloa, Mexico
Hybrid Gold, Green Published
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