Roughness and wettability of titanium implant surfaces modify the salivary pellicle composition
Por:
Martinez-Hernandez, Miryam, Hannig, Matthias, Garcia-Perez, I, Victor, Olivares-Navarrete, Rene, Fecher-Trost, Claudia, Almaguer-Flores, Argelia
Publicada:
1 jul 2021
Ahead of Print:
1 nov 2020
Resumen:
This study reports the differences in the protein composition of
salivary pellicles formed under in situ conditions on two Titanium (Ti)
surfaces, with different roughness and wettability. Smooth pretreatment
Ti surfaces (Ti-PT) with an average roughness (Ra) of 0.45 mu m and a
water contact angle (WCA) of 92.4 degrees, as well as a more rough
sandblasted, large grit, acid-etched treatment Ti surfaces (Ti-SLA) with
a Ra of 3.3 mu m and WCA of 131.8 degrees, were tested. The salivary
pellicles were quantitatively analyzed by bicinchoninic acid assays, and
the protein identification was performed by Nano-LC-MS/MS (nano mass
spectrometry). Protein levels of 2.5, and 9.1 mu g/ml were quantified
from the detached salivary pellicle formed on the Ti-PT and Ti-SLA
surfaces, respectively. Using Nano-LC-MS/MS, a total of 597 proteins
were identified on all the substrates tested; 43 proteins were
identified only on the Ti-PT, and 226 proteins were adsorbed solely on
the Ti-SLA substrates. The physicochemical characteristics of the Ti
implant surfaces modified the amount and the identity of the salivary
proteome of the pellicles formed, confirming the high selectivity of the
protein pellicle formed on a surface once is exposed in the oral cavity.
Filiaciones:
Garcia-Perez, Victor, I , Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Circuito Exterior S-N,Ciudad Univ,CP 04510, CDMX, Mexico
Saarland Univ, Univ Hosp, Clin Operat Dent Periodontol & Prevent Dent, Homburg Saar, Germany
Virginia Commonwealth Univ, Sch Engn, Dept Biomed Engn, Richmond, VA USA
Saarland Univ, Inst Expt & Clin Pharmacol & Toxicol, Homburg Saar, Germany
|