EFFECTS OF WATER RESTRICTION ON CARBOHYDRATES CONCENTRATION, STARCH GRANULES SIZE AND AMYLOLYTIC ACTIVITY IN SEEDS OF PHASEOLUS VULGARIS L. AND P. ACUTIFOLIUS A. GRAY
Por:
Cilia Garcia, Marcela, Pena-Valdivia, Cecilia B., Bernal Gracida, Lilia A., Yanez Jimenez, Petra, Garcia Esteva, Antonio, Padilla-Chacon, Daniel
Publicada:
1 abr 2021
Categoría:
Plant science
Resumen:
Background: Seed mass is a key component of adaptation in plants that
are strongly affected by development and maturity, and, at the same
time, all is modulated by the environment of cultivation.
Hypotheses: The response to water deficit in seeds of P. vulgaris L. and
P. acutifolius A. Gray is species-dependent and affects their
biochemical and morphological characteristics.
Studied species: The studied cultivars were Rosa Bufa (P. vulgaris) and
cv. 10017 (P. acutifolius). Seeds were obtained from plants grown at 100
% and 25 % soil field capacity during their pod development.
Study site and dates: The experiments were performed during May and
August 2018 in a greenhouse at the Colegio de Postgraduados, Texcoco,
State of Mexico (altitude 2,353 m).
Methods: The biochemical and morphological characteristics and the grain
size of starch in seeds cotyledons were assessed.
Results: Water restriction had no significant effects on the seed
thickness, width, or mass only length decreased in P. acutifolius. In
both species, the axis size of the starch granules decreased due to the
stress, glucose concentration increased, sucrose and starch were not
altered. Water imbibition increased six times in P. vulgaris seeds with
no effect on the germination. The a-amylase activity was 25 - 35 %
lower in both species due to the water restriction, particularly in P.
acutifolius the activity was two-fold higher than in P. vulgaris.
Conclusions: Comparing the carbohydrate concentration in germinating
seeds of common and Tepary beans gave insights on the nutrient reserves
mobilization during seed maturation and germination.
Filiaciones:
Cilia Garcia, Marcela:
Colegio Postgrad, Programa Posgrad Bot, Texcoco, Edo Mex, Mexico
Pena-Valdivia, Cecilia B.:
Colegio Postgrad, Programa Posgrad Bot, Texcoco, Edo Mex, Mexico
Bernal Gracida, Lilia A.:
Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Quim, Ciudad Univ, Mexico City, DF, Mexico
Yanez Jimenez, Petra:
Colegio Postgrad, Programa Posgrad Bot, Texcoco, Edo Mex, Mexico
Garcia Esteva, Antonio:
Colegio Postgrad, Programa Posgrad Bot, Texcoco, Edo Mex, Mexico
Padilla-Chacon, Daniel:
CONACyT, Colegio Postgrad, Programa Posgrad Bot, Texcoco, Edo Mex, Mexico
Gold, All Open Access; Gold
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