Assessing the Diversity of Benthic Sulfate-Reducing Microorganisms in Northwestern Gulf of Mexico by Illumina Sequencing of dsrB Gene
Por:
Sanchez-Soto, Ma. Fernanda, Cerqueda-Garcia, Daniel, Alcantara-Hernandez, Rocio J., Falcon, Luisa I., Pech, Daniel, Arcega-Cabrera, Flor, Aguirre-Macedo, Ma. Leopoldina, Garcia-Maldonado, Jose Q.
Publicada:
1 may 2021
Resumen:
This study investigates the community composition, structure, and
abundance of sulfate-reducing microorganisms (SRM) in surficial
sediments of the Northwestern Gulf of Mexico (NWGoM) along a bathymetric
gradient. For these purposes, Illumina sequencing and quantitative PCR
(qPCR) of the dissimilatory sulfite reductase gene beta subunit (dsrB
gene) were performed. Bioinformatic analyses indicated that SRM
community was predominantly composed by members of Proteobacteria and
Firmicutes across all the samples. However, Actinobacteria,
Thermodesulfobacteria, and Chlorobi were also detected. Phylogenetic
analysis indicated that unassigned dsrB sequences were related to
Deltaproteobacteria and Nitrospirota superclusters, Euryarchaeota, and
to environmental clusters. PCoA ordination revealed that samples
clustered in three different groups. PERMANOVA indicated that water
depth, temperature, redox, and nickel and cadmium content were the main
environmental drivers for the SRM communities in the studied sites.
Alpha diversity and abundance of SRM were lower for deeper sites,
suggesting decreasing sulfate reduction activity with respect to water
depth. This study contributes with the understanding of distribution and
composition of dsrAB-containing microorganisms involved in sulfur
transformations that may contribute to the resilience and stability of
the benthic microbial communities facing metal and hydrocarbon pollution
in the NWGoM, a region of recent development for oil and gas drilling.
Filiaciones:
Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Recursos Mar, Merida, Yucatan, Mexico
Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Consorcio Invest Golfo Mexico CIGOM, Merida, Yucatan, Mexico
Univ Nacl Autonoma Mexico, Inst Geol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Ecol, Parque Cient & Tecnol Yucatan, Sierra Papacal, Mexico
El Colegio Frontera Sur, Lab Biodivers Marina & Cambio Climat, Campeche, Mexico
Univ Nacl Autonoma Mexico, Fac Quim, Unidad Quim Sisal, Sisal, Yucatan, Mexico
Inst Politecn Nacl, CONACYT, Ctr Invest & Estudios Avanzados, Merida, Yucatan, Mexico
|