Deconvoluting apocarotenoid-mediated retrograde signaling networks regulating plastid translation and leaf development
Por:
Escobar-Tovar, Lina, Sierra, Julio, Hernandez-Munoz, Arihel, McQuinn, Ryan P., Mathioni, Sandra, CORDOBA, ELIZABETH, Colas des Francs-Small, Catherine, Meyers, Blake C., Pogson, Barry, LEON, PATRICIA
Publicada:
1 mar 2021
Resumen:
Signals originating within plastids modulate organelle differentiation
by transcriptionally regulating nuclear-encoded genes. These retrograde
signals are also integral regulators of plant development, including
leaf morphology. The clb5 mutant displays severe leaf morphology defects
due to Apocarotenoid Signal 1 (ACS1) accumulation in the developmentally
arrested plastid. Transcriptomic analysis of clb5 validates that ACS1
accumulation deregulates hundreds of nuclear genes, including the
suppression of most genes encoding plastid ribosomal proteins. Herein,
we order the molecular events causing the leaf phenotype associated with
the accumulation of ACS1, which includes two consecutive retrograde
signaling cascades. Firstly, ACS1 originating in the plastid drives
inhibition of plastid translation (IPT) via nuclear transcriptome
remodeling of chlororibosomal proteins, requiring light as an essential
component. Subsequently, IPT results in leaf morphological defects via a
GUN1-dependent pathway shared with seedlings undergoing chemical IPT
treatments and is restricted to an early window of the leaf development.
Collectively, this work advances our understanding of the complexity
within plastid retrograde signaling exemplified by sequential signal
exchange and consequences that in a particular temporal and spatial
context contribute to the modulation of leaf development.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA
Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA
Australian Natl Univ, Res Sch Biol, Australian Res Council, Ctr Excellence Plant Energy Biol, Canberra, ACT 0200, Australia
Univ Western Australia, Sch Mol Sci, Australian Res Council, Ctr Excellence Plant Energy Biol, Crawley, WA 6009, Australia
|